, Volume 89, Issue 1–2, pp 83–89 | Cite as

Effects of streptomycin on diploid tobacco callus cultures and the isolation of resistant mutants

  • Nakdimon Umiel
  • Rina Goldner


Diploid callus cultures from four genetic lines of tobacco (Nicotiana tabacum) were grown in the presence of 0–2 mg/ml streptomycin sulfate. These genetic lines are closely related with regard to nuclear genes, but differ in their respective cytoplasmic genomes. In calli from all these four lines, the formation of green color was inhibited at 0.5 mg/ml streptomycin. Small differences were observed between the four lines in their respective growth on medium containing streptomycin. Streptomycin resistant mutants were isolated from cultures at high drug concentrations, using as criteria for isolation either the ability of the callus to grow green, or a better rate of growth as indicated by the size of white callus.


Streptomycin Small Difference Drug Concentration Nuclear Gene Green Color 
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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Nakdimon Umiel
    • 1
  • Rina Goldner
    • 1
  1. 1.Department of Field and Vegetable CropsThe Hebrew University, Faculty of AgricultureRehovotIsrael

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