Effects of streptomycin on diploid tobacco callus cultures and the isolation of resistant mutants
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Diploid callus cultures from four genetic lines of tobacco (Nicotiana tabacum) were grown in the presence of 0–2 mg/ml streptomycin sulfate. These genetic lines are closely related with regard to nuclear genes, but differ in their respective cytoplasmic genomes. In calli from all these four lines, the formation of green color was inhibited at 0.5 mg/ml streptomycin. Small differences were observed between the four lines in their respective growth on medium containing streptomycin. Streptomycin resistant mutants were isolated from cultures at high drug concentrations, using as criteria for isolation either the ability of the callus to grow green, or a better rate of growth as indicated by the size of white callus.
KeywordsStreptomycin Small Difference Drug Concentration Nuclear Gene Green Color
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