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Comparison of the redox activities in plasma membranes from roots and shoots of etiolated bean seedlings

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Summary

Plasma membrane (PM) vesicles were purified in parallel from the roots and shoots of 6-day-old etiolated bean (Phaseolus vulgaris L.) seedlings, grown in water culture at 25 °C, by aqueous polymer two-phase partitioning. The purity of PM fractions was determined by measuring the activity of known marker enzymes (vanadate-sensitive Mg-ATPase, 1,3-β-glycan synthase, latent ID-Pase, cytochromec oxidase, and antimycin-A-insensitive cytochromec reductase). NADH-(acceptor) oxidoreductase activities were determined with the following electron acceptors: ferricyanide, cytochromec, duroquinone, monodehydroascorbate, Fe3+-EDTA, and oxygen. Cytochromeb content was also determined. In general, results show that redox activities are higher in the root PM than in the shoot PM which follows the glycan synthase II (PM marker) pattern. The relative activities of the distinct redox enzymes measured (activity profile) are remarkably similar in the root and shoot PM preparations. The cytochromeb content and level of ascorbate reduction were also similar in both plant organs. However, the ratio of NADH-(acceptor) oxidoreductase activity to cytochrome content was signifcantly higher in roots when compared to the shoots.

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Abbreviations

CCO:

cytochromec oxidase

CCR:

cytochromec reductase

GSII:

1,3-β-glycan synthase

MF:

microsomal fraction

N-CC-OR:

NADH-cytochromec oxidoreductase

N-DQ-OR:

NADH-duroquinone oxidoreductase

N-FC-OR:

NADH-ferricyanide oxidoreductase

N-FE-OR:

NADH-Fe3+-EDTA oxidoreductase

N-MDA-OR:

NADH-monodehydroascorbate oxidoreductase

PM:

plasma membrane

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Authors and Affiliations

  1. Department of Biology, University of Antwerp, Groenenborgerlaan 171, B-2020, Antwerp, Belgium

    H. Asard

  2. Institute of Biophysics, BRC, Hungarian Academy of Sciences, Szeged

    A. Bérczi

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  1. H. Asard
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  2. A. Bérczi
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Asard, H., Bérczi, A. Comparison of the redox activities in plasma membranes from roots and shoots of etiolated bean seedlings. Protoplasma 205, 37–42 (1998). https://doi.org/10.1007/BF01279291

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  • DOI: https://doi.org/10.1007/BF01279291

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