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Proteins reacting with cadherin and catenin antibodies are present in maize showing tissue-, domain-, and development-specific associations with endoplasmic-reticulum membranes and actin microfilaments in root cells

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Summary

With heterologous antibodies raised against animal N-cadherin, α-catenin, and β-catenin, we have visualized their reactive proteins within cells of maize root apices. Embedding using Steedman's wax allowed us to accomplish tissue-specific analysis which revealed that cells of epidermis, endodermis/pericycle, and outer stele tissues, all of which are tightly associated to each other, are especially enriched with presumed plant homologues of N-cadherin and both catenins. In the root epidermis, trichoblasts initiating root hairs showed prominent accumulations of cadherin-like antigens at outgrowing domains where they co-localize with actin. Close associations of cadherin-like proteins with F-actin were detected in parenchymatic cells of the stele, also at the immunogold electron microscopy level. A possible role of these interesting proteins in membrane-membrane interactions is indicated by their prominent accumulations at endoplasmic-reticulum-enriched pit-field-based plant cell adhesion domains in plasmolyzing cells of maize root apices exposed to mannitol. Intriguingly, these unique adhesion domains of plasmolyzing cells are enriched with endoplasmic-reticulum-resident calreticulin. Cadherin-like, but not catenin-like, proteins were abundant also within the nucleoplasm.

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Abbreviations

AGPs:

arabinogalactan proteins

EM:

electron microscopy

ER:

endoplasmic reticulum

MFs:

microfilaments

SB:

stabilizing buffer

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Authors and Affiliations

  1. Botanisches Institut, Rheinische Friedrich-Wilhelms-Universität Bonn, Kirschallee 1, D-53115, Bonn, Federal Republic of Germany

    F. Baluška, J. Šamaj & D. Volkmann

  2. Institute of Botany, Slovak Academy of Sciences, Bratislava

    F. Baluška

  3. Institute of Plant Genetics, Slovak Academy of Sciences, Nitra

    J. Šamaj

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  1. F. Baluška
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  2. J. Šamaj
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  3. D. Volkmann
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Baluška, F., Šamaj, J. & Volkmann, D. Proteins reacting with cadherin and catenin antibodies are present in maize showing tissue-, domain-, and development-specific associations with endoplasmic-reticulum membranes and actin microfilaments in root cells. Protoplasma 206, 174–187 (1999). https://doi.org/10.1007/BF01279265

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  • DOI: https://doi.org/10.1007/BF01279265

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