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Diurnal changes in xylem pressure and mesophyll cell turgor pressure of the lianaTetrastigma voinierianum: The role of cell turgor in long-distance water transport

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Summary

Long-term xylem pressure measurements were performed on the lianaTetrastigma voinierianum (grown in a tropical greenhouse) between heights of 1 m and 9.5 m during the summer and autumn seasons with the xylem pressure probe. Simultaneously, the light intensity, the temperature, and the relative humidity were recorded at the measuring points. Parallel to the xylem pressure measurements, the diurnal changes in the cell turgor and the osmotic pressure of leaf cells at heights of 1 m and 5 m (partly also at a height of 9.5 m) were recorded. The results showed that tensions (and height-varying tension gradients) developed during the day time in the vessels mainly due to an increase in the local light intensity (at a maximum 0.4 MPa). The decrease of the local xylem pressure from positive, subatmospheric or slightly above-atmospheric values (established during the night) to negative values after daybreak was associated with an almost 1 ∶ 1 decrease in the cell turgor pressure of the mesophyll cells (on average from about 0.4 to 0.5 MPa down to 0.08 MPa). Similarly, in the afternoon the increase of the xylem pressure towards more positive values correlated with an increase in the cell turgor pressure (ratio of about 1 ∶ 1). The cell osmotic pressure remained nearly constant during the day and was about 0.75–0.85 MPa between 1 m and 9.5 m (within the limits of accuracy). These findings indicate that the turgor pressure primarily determines the corresponding pressure in the vessels (and vice versa) due to the tight hydraulic connection and thus due to the water equilibrium between both compartments. An increase in the transpiration rate (due to an increase in light intensity) results in very rapid establishment of a new equilibrium state by an equivalent decrease in the xylem and cell turgor pressure. From the xylem, cell turgor, and cell osmotic pressure data the osmotic pressure (or more accurately the water activity) of the xylem sap was calculated to be about 0.35–0.45 MPa; this value was apparently not subject to diurnal changes. Considering that the xylem pressure is determined by the turgor pressure (and vice versa), the xylem pressure of the liana could not drop to — in agreement with the experimental results — less than -0.4 MPa, because this pressure corresponds to zero turgor pressure.

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References

  • Andrews FC (1976) Colligative properties of simple solutions. Science 194: 567–571

    Google Scholar 

  • Balling A, Zimmermann U (1990) Comparative measurements of the xylem pressure ofNicotiana plants by means of the pressure bomb and pressure probe. Planta 182: 325–338

    Google Scholar 

  • — —, Büchner K-H, Lange OL (1988) Direct measurement of negative pressure in artificial-biological systems. Naturwissenschaften 75: 409–411

    Google Scholar 

  • Barker M, Becker P (1995) Sap flow rate and sap nutrient content of a tropical rain forest canopy species,Dryobalanops aromatica, in Brunei. Selbyana 16: 201–211

    Google Scholar 

  • Benkert R, Zhu J-J, Zimmermann G, Türk R, Bentrup F-W, Zitnmermann U (1995) Long-term xylem pressure measurements in the lianaTetrastigma voinierianum by means of the xylem pressure probe. Planta 196: 804–813

    Google Scholar 

  • Bode HR (1923) Beiträge zur Dynamik der Wasserbewegung in den Gefäßpflanzen. Jahrb Wiss Bot 62: 92–127

    Google Scholar 

  • Braun HJ (1984) The significance of the accessory tissues of the hydrosystem for osmotic water shifting as the second principle of water ascent, with some thoughts concerning the evolution of trees. IAWA Bull 5: 275–294

    Google Scholar 

  • Canny MJ (1991) The xylem wedge as a functional unit: speculations on the consequences of flow in leaky tubes. New Phytol 118: 367–374

    Google Scholar 

  • — (1993) The transpiration stream in the leaf apoplast: water and solutes. Philos Trans R Soc Lond Biol Sci 341: 87–100

    Google Scholar 

  • — (1995a) Potassium cycling inHelianthus: ions of the xylem sap and secondary vessel formation. Philos Trans R Soc Lond Biol Sci 348: 457–469

    Google Scholar 

  • — (1995b) A new theory for the ascent of sap: cohesion supported by tissue pressure. Ann Bot 75: 343–357

    Google Scholar 

  • Dainty J (1963) Water relations of plant cells. Adv Bot Res 1: 279–326

    Google Scholar 

  • Ferguson AR, Eiseman JA, Dale JR (1981) Xylem sap fromActinidia chinensis: gradients in sap composition. Ann Bot 48: 75–80

    Google Scholar 

  • Holbrook NM, Burns MJ, Field CB (1995) Negative xylem pressures in plants: a test of the balancing pressure technique. Science 270: 1193–1194

    Google Scholar 

  • Huck MG, Klepper B, Taylor HM (1970) Diurnal variations in root diameter. Plant Physiol 45: 529–530

    Google Scholar 

  • Jachetta JJ, Appleby AP, Boersma L (1986) Use of the pressure vessel to measure concentrations of solutes in apoplastic and membrane-filtered symplastic sap in sunflower leaves. Plant Physiol 82: 995–999

    Google Scholar 

  • Katchalsky A, Curran PF (1965) Nonequilibrium thermodynamics in biophysics. Havard University Press, Cambridge, Mass

    Google Scholar 

  • Klepper B, Browning VD, Taylor HM (1971) Stem diameter in relation to plant water status. Plant Physiol 48: 683–685

    Google Scholar 

  • Kramer PJ (1983) Water relations of plants. Academic Press, New York

    Google Scholar 

  • MacDougal DT (1924) Dendrographic measurements. Carnegie Inst Washington Publ 350: 1–88

    Google Scholar 

  • Malone M (1993) Hydraulic signals. Philos Trans R Soc Lond Biol Sci 341: 33–39

    Google Scholar 

  • —, Leigh RA, Tomos AD (1989) Extraction and analysis of sap from individual wheat leaf cells: the effect of sampling speed on the osmotic pressure of extracted sap. Plant Cell Environ 12: 919–926

    Google Scholar 

  • Marienfeld S, Zhu JJ, Koyro H-W, Schröder WH, Zimmermann U (1996) Direkte Probenentnahme aus dem Xylem: Vergleich mit konventionell gewonnenen Proben. In: Abstracts of the Botanikertagung, Düsseldorf, p 325

  • Mauro A (1965) Osmotic flow in a rigid porous membrane. Science 149: 867–869

    Google Scholar 

  • — (1981) The role of negative pressure in osmotic equilibrium and osmotic flow. In: Ussing HH, Bindslev N, Lassen NA, Sten-Knudsen O (eds) Water transport across epithelia. Alfred Benzon Symp. 15. Munksgaard, Copenhagen, pp 107–119

    Google Scholar 

  • Milburn JA (1996) Sap ascent in vascular plants: challengers to the cohesion theory ignore the significance of immature xylem and the recycling of Munch water. Ann Bot 78: 399–407

    Google Scholar 

  • Moore PH, Cosgrove DJ (1991) Developmental changes in cell and tissue water relations parameters in storage parenchyma of sug-arcane. Plant Physiol 96: 794–801

    Google Scholar 

  • Passioura JB (1991) An impasse in plant water relations? Bot Acta 104: 405–411

    Google Scholar 

  • —, Munns R (1984) Hydraulic resistance of plants II: effects of rooting medium, and time of day, in barley and lupin. Aust J Plant Physiol 11: 341–350

    Google Scholar 

  • Pickard WF (1981) The ascent of sap in plants. Prog Biophys Mol Biol 37: 181–229

    Google Scholar 

  • Plumb RC, Bridgman WB (1972) Ascent of sap in trees. Science 176: 1129–1131

    Google Scholar 

  • Pockman WT, Sperry JS, O'Leary JW (1995) Sustained and significant negative water pressure in xylem. Nature 378: 715–716

    Google Scholar 

  • Renner O (1911) Experimentelle Beiträge zur Kenntnis der Wasserbewegung. Flora 113: 171–247

    Google Scholar 

  • Richter H (1997) Water relations of plants in the field: some comments on the measurement of selected parameters. J Exp Bot 48: 1–7

    Google Scholar 

  • Schill V, Hartung W, Orthen B, Weisenseel MH (1996) The xylem sap of maple (Acer platanoides) trees: sap obtained by a novel method shows changes with season and height. J Exp Bot 47: 123–133

    Google Scholar 

  • Schneider H, Wistuba N, Miller B, Geßner P, Thürmer F, Melcher P, Meinzer F, Zimmermann U (1997a) Diurnal variation in the radial reflection coefficient of intact maize roots determined with the xylem pressure probe. J Exp Bot 48: 2045–2053

    Google Scholar 

  • —, Zhu JJ, Zimmermann U (1997b) Xylem and cell turgor pressure probe measurements in intact roots of glycophytes: transpiration induces a change in the radial and cellular reflection coefficients. Plant Cell Environ 20: 221–229

    Google Scholar 

  • Scholander PF, Hammel HT, Bradstreet ED, Hemmingsen EA (1965) Sap pressure in vascular plants. Science 148: 339–346

    Google Scholar 

  • Shackel K (1996) To tense, or not too tense: reopening the debate about water ascent in plants. Trends Plant Sci 1: 105–106

    Google Scholar 

  • Stahlberg R, Cosgrove DJ (1995) Comparison of electric and growth responses to excision in cucumber and pea seedlings II: long-distance effects are caused by the release of xylem pressure. Plant Cell Environ 18: 33–41

    Google Scholar 

  • Steudle E (1989) Water flow in plants and its coupling to other processes: an overview. Methods Enzymol 174: 183–225

    Google Scholar 

  • — (1992) The biophysics of plant water: compartmentation, coupling with metabolic processes and flow of water in plant roots. In: Somero GN, Osmond CB, Bolis CL (eds) Water and life: comparative analysis of water relationships at the organismic, cellular, and molecular levels. Springer, Berlin Heidelberg New York Tokyo, pp 173–204

    Google Scholar 

  • Turner NC, Spurway RA, Schulze E-D (1984) Comparison of water potentials measured by in situ psychrometry and pressure chamber in morphologically different species. Plant Physiol 74: 316–319

    Google Scholar 

  • Wei C, Steudle E, Tyree MT (1998) Xylem pressure in maize leaves measured with a cell pressure probe: a test of the Cohesion-Tension theory. J Exp Bot 49 Suppl: 10

    Google Scholar 

  • Zimmermann MH (1983) Xylem structure and the ascent of sap. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Zimmermann U (1978) Physics of turgor- and osmoregulation. Annu Rev Plant Physiol 29: 121–148

    Google Scholar 

  • — (1989) Water relations of plant cells: pressure probe technique. Methods Enzymol 174: 338–366

    Google Scholar 

  • —, Räde H, Steudle E (1969) Kontinuierliche Druckmessung in Pflanzenzellen. Naturwissenschaften 56: 634

    Google Scholar 

  • —, Rygol J, Balling A, Klöck G, Metzler A, Haase A (1992) Radial turgor and osmotic pressure profiles in intact and excised roots ofAster tripolium. Plant Physiol 99: 186–196

    Google Scholar 

  • —, Haase A, Langbein D, Meinzer F (1993) Mechanisms of longdistance water transport in plants: a re-examination of some paradigms in the light of new evidence. Philos Trans R Soc Lond Biol Sci 341: 19–31

    Google Scholar 

  • —, Meinzer FC, Benkert R, Zhu JJ, Schneider H, Goldstein G, Kuchenbrod E, Haase A (1994a) Xylem water transport: is the available evidence consistent with the cohesion theory? Plant Cell Environ 17: 1169–1181

    Google Scholar 

  • —, Zhu JJ, Meinzer PC, Goldstein G, Schneider H, Zimmermann G, Benkert R, Thürmer F, Melcher P, Webb D, Haase A (1994b) High molecular weight organic compounds in the xylem sap of mangroves: implications for long-distance water transport. Bot Acta 107: 218–229

    Google Scholar 

  • —, Bentrup F-W, Haase A (1995a) Xylem pressure, flow, and sap composition of trees determined by means of the xylem pressure probe. In: Terazawa M, McLeod CA, Tamai Y (eds) Tree sap. Hokkaido University Press, Sapporo-shi, pp 47–58

    Google Scholar 

  • —, Meinzer F, Bentrup F-W (1995b) How does water ascend in tall trees and other vascular plants? Ann Bot 76: 545–551

    Google Scholar 

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Authors and Affiliations

  1. Lehrstuhl für Biotechnologie, Universität Würzburg, Würzburg

    F. Thürmer, J. J. Zhu, H. Schneider, R. Benkert, P. Geßner, B. Herrmann & U. Zimmerniann

  2. Institut für Pflanzenphysiologie, Universität Salzburg, Hellbrunnerstraße 34, A-5020, Salzburg, Austria

    N. Gierlinger & F. -W. Bentrup

Authors
  1. F. Thürmer
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  2. J. J. Zhu
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  3. N. Gierlinger
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  4. H. Schneider
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  5. R. Benkert
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  6. P. Geßner
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  7. B. Herrmann
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  8. F. -W. Bentrup
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  9. U. Zimmerniann
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Correspondence to F. -W. Bentrup.

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Thürmer, F., Zhu, J.J., Gierlinger, N. et al. Diurnal changes in xylem pressure and mesophyll cell turgor pressure of the lianaTetrastigma voinierianum: The role of cell turgor in long-distance water transport. Protoplasma 206, 152–162 (1999). https://doi.org/10.1007/BF01279262

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  • DOI: https://doi.org/10.1007/BF01279262

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