Summary
This electron microscope study was undertaken to test the prediction made from an indirect assay method for mitotic centers (centrioles), that chloramphenicol inhibits centriole replication during first cleavage division in the eggs of the sand dollar,Dendraster excentricus. Extensive serial sectioning through both untreated and chloramphenicol-treated eggs, coupled with thorough examination of these sections, has demonstrated that 57% of the untreated eggs and 14% of the chloramphenicol-treated eggs contained paired centrioles at a time when centriole pairs normally exist. This study thus gives direct evidence that chloramphenicol inhibits centriole replication.
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Deutch, A.H., Shumway, L.K. Centriole behavior in chloramphenicol-treated eggs of the sand dollar,Dendraster excentricus . Protoplasma 76, 387–402 (1973). https://doi.org/10.1007/BF01279135
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DOI: https://doi.org/10.1007/BF01279135