Skip to main content
Log in

Molecular control of early cone development inPinus radiata

  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

A family of genes expressed during early stages of shoot development were isolated fromPinus radiata. A homologue of theLEAFY/FLORICAULA flower meristem-identity genes,NEEDLY (NLY), and three MADS-box genes,PrMADS1, PrMADS2 andPrMADS3 (Pinus radiata MADS-box genes), were expressed at early stages of initiation and differentiation of reproductive (male and female) cone buds, as well as vegetative buds. Expression ofNLY in transgenicArabidopsis thaliana promoted floral fate, demonstrating that it encodes a functional ortholog of theFLORICAUL A/LEAFY genes of angiosperms.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

DSB:

dwarf shoot bud

LSTB:

long-shoot terminal bud

PCB:

pollen cone bud

SCB:

seed cone bud

LD:

long day

SD:

short day

References

  • Blázquez MA, Soowal LN, Lee I, Weigel D (1997)LEAFY expression and flower initiation inArabidopsis. Development 124: 3835–3844

    PubMed  Google Scholar 

  • Cacharrón J, Fischer A, Saedler H, Theißen G (1995) Expression patterns of MADS-box genes as studied by in situ hybridisation. Maize Genet Coop Newslett 69: 37–38

    Google Scholar 

  • Cardon GH, Frey M, Saedler H, Gierl A (1993) Mobility of the maize transposable elementEn/Spm inArabidopsis thaliana. Plant J 3: 773–784

    PubMed  Google Scholar 

  • Chung Y-Y, Kim S-R, Finkel D, Yanofsky MF, An G (1994) Early flowering and reduced apical dominance result from ectopic expression of a rice MADS box gene. Plant Mol Biol 26: 657–665

    PubMed  Google Scholar 

  • Coen E, Romero J, Doyle S, Elliot R, Murphy G, Carpenter R (1990)floricaula: a homeotic gene required for flower development inAntirrhinum majus. Cell 63: 1311–1322

    PubMed  Google Scholar 

  • Davies B, Schwarz-Sommer Z (1994) Control of floral organ identity by homeotic MADS-box transcription factors. In: Novel L (ed) Results and problems in cell differentiation: plant promoters and transcription factors. Springer, Berlin Heidelberg New York Tokyo, pp 235–258

    Google Scholar 

  • Flanagan CA, Ma H (1994) Spatially and temporally regulated expression of the MADS-box geneAGL2 in wild-type and mutantArabidopsis flowers. Plant Mol Biol 26: 581–595

    PubMed  Google Scholar 

  • Harrison D, Slee M (1992) Long shoot terminal bud development and the differentiation of pollen- and seed-cone buds inPinus caribaeva var. hondurensis. Can J For Res 22: 1656–1668

    Google Scholar 

  • Hofer J, Turner L, Hellens R, Ambrose M, Matthews P, Michael A, Ellis N (1997)UNIFOLIATA regulates leaf and flower morphogenesis in pea. Curr Biol 7: 581–588

    PubMed  Google Scholar 

  • Huang H, Tudor M, Weiss C, Hu Y, Ma H (1995) TheArabidopsis MADS-box geneAGL3 is widely expressed and encodes a sequence-specific DNA-binding protein. Plant Mol Biol 28: 549–567

    PubMed  Google Scholar 

  • Ingram GC, Goodrich J, Wilkinson MD, Simon R, Haughn GW, Coen ES (1995) Parallels betweenUNUSUAL FLORAL ORGANS andFIMBRIATA, genes controlling flower development inArabidopsis andAntirrhinum. Plant Cell 7: 1501–1510

    PubMed  Google Scholar 

  • Jofuku KD, der Boer BGW, Van Montagu M, Okamuro JK (1994) Control ofArabidopsis flower and seed development by the homeotic geneAPETALA2. Plant Cell 6: 1211–1225

    PubMed  Google Scholar 

  • Kelly AJ, Bonnlander MB, Meeks-Wagner DR (1995)NFL, the tobacco homolog ofFLORICAULA andLEAFY, is transcriptionally expressed in both vegetative and floral meristems. Plant Cell 7: 225–234

    PubMed  Google Scholar 

  • Kempin SA, Savidge B, Yanofsky MF (1995) Molecular basis of thecauliflower phenotype inArabidopsis. Science 267: 522–525

    PubMed  Google Scholar 

  • Liu J-J, Podila GK (1997) Characterisation of a MADS-box gene (Y09611) from immature female cones of red pine. Plant Physiol 113: 665

    Google Scholar 

  • Lu Z-X, Wu M, Loh C-S, Yeong C-Y, Goh C-J (1993) Nucleotide sequence of a flower-specific MADS-box cDNA clone from orchid. Plant Mol Biol 23: 901–904

    PubMed  Google Scholar 

  • Ma H (1994) The unfolding drama of flower development: recent results from genetic and molecular analyses. Genes Dev 8: 745–756

    PubMed  Google Scholar 

  • —, Yanofsky MF, Meyerowitz EM (1991)AGL1–AGL6, anArabidopsis gene family with similarity to floral homeotic and transcription factor genes. Genes Dev 5: 484–495

    PubMed  Google Scholar 

  • Mandel MA, Yanofsky MF (1995) A gene triggering flower formation inArabidopsis. Nature 377: 522–524

    PubMed  Google Scholar 

  • —, Gustafson-Brown C, Savidge B, Yanofsky ME (1992) Molecular characterisation of theArabidopsis floral homeotic geneAPETALA. Nature 360: 273–277

    PubMed  Google Scholar 

  • Mena M, Mandel MA, Lerner DR, Yanofsky MF, Schmidt RJ (1995) A characterisation of the MADS-box gene family in maize. Plant J 8: 845–854

    PubMed  Google Scholar 

  • Mouradov A, Glassick T, Hamdorf B, Murphy L, Gale B, Marla S, Teasdale RD (1998a)NEEDLY, aPinus radiata ortholog ofFLORICAULA/LEAFY genes, expressed in both reproductive and vegetative meristems. Proc Natl Acad Sci USA 95: 6537–6542

    PubMed  Google Scholar 

  • —, Glassick T, Hamdorf B, Murphy L, Marla S, Yang Y, Teasdale RD (1998b) MADS-box genes, expressed early in male and female reproductive structures of Monterey pine. Plant Physiol 117: 55–62

    PubMed  Google Scholar 

  • Münster T, Pahnke J, Di Rosa A, Kim JT, Matin W, Saedler H, Theißen G (1997) Floral homeotic genes were recruited from homologous MADS-box genes preexisting in the common ancestor of ferns and seed plants. Proc Natl Acad Sci USA 94: 2415–2420

    PubMed  Google Scholar 

  • Rounsley S, Ditta G, Yanofsky MF (1995) Diverse roles of MADS-box genes inArabidopsis development. Plant Cell 7: 1259–1269

    PubMed  Google Scholar 

  • Savidge B, Rounsley SD, Yanofsky MF (1995) Temporal relationship between the transcription of twoArabidopsis MADS-box genes and the floral organ identity genes. Plant Cell 7: 721–733

    PubMed  Google Scholar 

  • Tandre K, Albert VA, Sundas A, Engström P (1995) Conifer homologues to genes that control floral development in angiosperms. Plant Mol Biol 27: 69–87

    PubMed  Google Scholar 

  • Theißen G, Kim JT, Saedler H (1996) Classification and phylogeny of the MADS-box multigene family suggest defined roles of MADS-box gene families in the morphological evolution of eukaryotes. J Mol Evol 43: 484–516

    PubMed  Google Scholar 

  • Weigel D, Nilsson O (1995) A developmental switch sufficient for flower initiation in diverse plants. Nature 377: 495–500

    PubMed  Google Scholar 

  • —, Alvarez J, Smyth DR, Yanofsky MF, Meyerowitz EM (1992) LEAFY controls floral meristem identity inArabidopsis. Cell 69: 843–859

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mouradov, A., Glassick, T., Hamdorf, B. et al. Molecular control of early cone development inPinus radiata . Protoplasma 208, 3–12 (1999). https://doi.org/10.1007/BF01279069

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01279069

Keywords

Navigation