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Similar effects of glycine, zinc and an oxidizing agent on [3H]dizocilpine binding to the N-methyl-D-aspartate receptor in neocortical tissue from suicide victims and controls

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Summary

This study used [3H]dizocilpine (MK-801) binding to the N-methyl-D-aspartate (NMDA) receptor to examine glycine, redox and zinc modulatory sites in membranes derived from the frontal and parietal cortex of control subjects (n=8) and suicide victims (n=6). [3H]dizocilpine binding in the presence of glutamate and glutamate plus glycine was similar in control and suicide subjects. The sulphydryl redox site was assessed using the oxidizing agent 5,5′-dithio-bis (2-nitrobenzoic acid), which inhibited binding in a dosedependent fashion. Both redox and zinc sites were unaffected in the frontal and parietal cortex of suicide victims. These data indicate that the NMDA receptor and its glycine, redox and zinc subsites are preserved in the neocortex of suicide victims.

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Author notes

  1. A. M. Palmer

    Present address: Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

Authors and Affiliations

  1. Department of Psychiatry, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    M. A. Burns, V. Arango & J. J. Mann

  2. Department of Pharmacology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA

    A. M. Palmer

Authors
  1. A. M. Palmer
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  2. M. A. Burns
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  3. V. Arango
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  4. J. J. Mann
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Palmer, A.M., Burns, M.A., Arango, V. et al. Similar effects of glycine, zinc and an oxidizing agent on [3H]dizocilpine binding to the N-methyl-D-aspartate receptor in neocortical tissue from suicide victims and controls. J. Neural Transmission 96, 1–8 (1994). https://doi.org/10.1007/BF01277923

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  • DOI: https://doi.org/10.1007/BF01277923

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