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La maturation du fruit de tomate cerise: Mise en évidence, par cryodécapage, de l'évolution des chloroplastes en deux types de chromoplastes

The maturation of the cherry tomato fruit: Evidence, by freeze-etched studies, of the evolution of chloroplasts in two classes of chromoplasts

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Summary

During the maturation of the fruit of “cherry” tomato, the chloroplasts evolve into two different kinds of chromoplasts. One of them, located in the jelly part of the pericarp, contains only globular inclusions of \-carotene. On the other hand, the outer part of the fruit contains chromoplasts which possess, in addition of such globules, voluminous sheets of lycopene, easily cleaved by freeze-etching. These types of inclusions both occur in the lumens of chloroplast thylakoids which have already lost their chlorophylls.

Résumé

Durant la maturation du fruit de tomate « cerise », les chloroplastes évoluent en deux types de chromoplastes. Les uns, localisés exclusivement dans la partie gélifiée du péricarpe, ne contiennent que des inclusions globoÏdes à \-caroténe. Les autres, situés dans la partie externe du fruit, possèdent, outre ces globules pigmentaires, de volumineuses plaques cristallines de lycopène, aisément clivables par cryodécapage. Ces deux types d'inclusions prennent naissance à l'intérieur des lumens d'ex-thylacoides chloroplastiques, qui ont en un premier temps perdu leurs pigments chlorophylliens.

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Bibliographie

  • Ben-Shaul, Y., andS. Klein, 1965: Development and structure of carotene bodies in carrot roots. Botan. Gaz.126, 79–85.

    Google Scholar 

  • —,H. T. Epstein, andJ. A. Schiff, 1965: Studies of chloroplast development inEuglena, 10: The return of the chloroplast to proplastid condition during dark adaptation. Canad. J. Bot.43, 129–136.

    Google Scholar 

  • —, andY. Naftali, 1969: The development and ultrastructure of lycopene bodies in chloroplasts ofLycopersicum esculentum. Protoplasma67, 333–336.

    Google Scholar 

  • —,T. Treffry, andS. Klein, 1968: Fine structure of carotene body development. J. Microscopie7, 265–274.

    Google Scholar 

  • Brooks, J., andP. K. Stumpf, 1966: Fat metabolism in higher plants. XXIX. Properties of a soluble fatty acid synthesizing system from lettuce chloroplasts. Arch. Biochem. Biophys.116, 108–116.

    PubMed  Google Scholar 

  • Buvat, R., 1958: Nouvelles observations sur l'appareil de Golgi dans les cellules des végétaux vasculaires. C. R. Acad. Sci.246, 2157–2160.

    Google Scholar 

  • —, 1963: Electron microscopy of plant protoplasm. Int. Rev. Cytol.14, 41.

    PubMed  Google Scholar 

  • Delaporte, N., etD. Laval-Martin, 1971 a: Analyse spectrophotométrique des chlorophylles et des pheophytinesa etb en milieu hydroacétonique. I. Détermination des extinctions molaires. Anal. Chim. Acta55, 415–424.

    Google Scholar 

  • —, 1971 b: Analyse spectrophotométrique des chlorophylles et des pheophytinesa etb en milieu hydroacétonique. II. Méthode cinétique de dosage. Anal. Chim. Acta55, 425–435.

    Google Scholar 

  • Deroche, M. E., 1969: Hétérogénéité des pigments liposolubles des chloroplastes de blé. Physiol. Vég.7, 335–389.

    Google Scholar 

  • Duranton, J., etM. Lefort-Tran, 1968: Différenciation structurale et teneurs en protéines et en pigments chlorophylliens au cours du reverdissement chezZea mays L. Bull. Soc. FranÇ. Physiol. Vég.14, 249–273.

    Google Scholar 

  • Eilam, Y., andS. Klein, 1962: The effect of light intensity and sucrose feeding on the fine structure in chloroplast and in chlorophyll content of etiolated leaves. J. Cell Biol.14, 169–183.

    PubMed  Google Scholar 

  • Eyme, J., 1971: La structure des plastes dans les parenchymes des coupes receptaculaires florales du pÊcher (Prunus persica) chez les variétés à chair blanche et à chair jaune. C. R. Acad. Sci. Paris272, 1232–1235.

    Google Scholar 

  • Faludi, D. A., L. Fridvalsky, andI. Gyursan, 1968: Pigment composition and plastid structure in leaves of carotenoid mutants of maize. Planta78, 184–195.

    Google Scholar 

  • Frey-Wyssling, A., undK. Mühlethaler, 1949: über den Feinbau der Chlorophyll-Körner. Vierteljahresschr. Naturforschung. Ges. (Zürich)94, 179.

    Google Scholar 

  • — undE. Kreutzer, 1958 a: Die submikroskopische Entwicklung der Chromoplasten in den Blüten vonRanunculus repens. Planta51, 104–114.

    Google Scholar 

  • Frey-Wyssling, A., andK. Mühlethaler, 1958 b: The submicroscopic development of chromoplasts in the fruit ofCapsicum annuum L. J. Ultrastruct. Res.1, 397–411.

    PubMed  Google Scholar 

  • —, andF. Schwegler, 1965: Ultrastructure of the chromoplasts in the carrot root. J. Ultrastruct. Res.13, 543–558.

    PubMed  Google Scholar 

  • Gerola, F. M., F. Cristofori etG. Dassu, 1960 a: Ricerche sulle infrastrutture delle cellule del mesofillo fogliare di piante sane e virosate di tabacco (Nicotiana tabacum). A. Piante sane. Caryologia13, 352–366.

    Google Scholar 

  • —, 1960 b: Ricerche sulle infrastrutture delle cellule del mesofillo fogliare di piante sane e virosate di tabacco (Nicotiana tabacumi. B. Piante virosate. Caryologia13, 367–378.

    Google Scholar 

  • Goodenough, W. V., andL. A. Staehelin, 1971: Structural differentiation of stacked and unstacked chloroplast membranes (Chlamydomonas reinbardii). J. Cell. Biol.48, 594–619.

    Google Scholar 

  • Grilli, M., 1965: Origine e sviluppo dei cromoplasti nei frutti di zucca americana (Cucurbita pepo L. cv.“small sugar”). II. Origine dei cromoplasti da cloroplasti e da proplastidi. Caryologia18, 435–459.

    Google Scholar 

  • GrÔnegress, P., 1971: The greening of chromoplasts inDaucus carota L. Planta98, 274–278.

    Google Scholar 

  • Hall, J. D., R. Barr, A. H. Abbas, andF. L. Crane, 1972: The ultrastructure of chloroplasts in mineral-deficient Maize-leaves. Plant Physiol.50, 404–409.

    Google Scholar 

  • Harris, W. M., 1968: Chromoplast development in ripening tomato fruits, plastid ultrastructure in relation to carotene content of three pigment lines. Diss. Abstr. Sect. B29, 1274.

    Google Scholar 

  • —, 1970: Chromoplasts of tomato fruits. II. The high-delta tomato. Bot. Gaz.131, 163–166.

    Google Scholar 

  • —, andA. R. Spurr, 1969 a: Chromoplasts of tomato fruits. II. The red tomato. Amer. J. Bot.56, 380–389.

    Google Scholar 

  • —, 1969 b: Chromoplasts of tomato fruits. IV. Ultrastructure of low-pigment and high-\ mutants. Carotene analyses. Amer. J. Bot.56, 369–374.

    Google Scholar 

  • Heitz, E., 1936: Untersuchungen über den Bau der Piastiden. I. Die gerichteten Chlorophyllscheiben der Chloroplasten. Planta26, 134–163.

    Google Scholar 

  • Hernandez-Gil, R., andM. Schaedle, 1973: Functional and structural changes in senescingPopulus deltoides (Bartr.) chloroplasts. Plant Physiol.51, 245–249.

    Google Scholar 

  • Khudairi, K. A., 1972: The ripening of tomatoes. A molecular ecological approach of the physiology of fruit ripening. Amer. Scientist60, 696–707.

    Google Scholar 

  • Klein, S., andL. Bogorad, 1964: Fine structure changes in proplastids during photodestruction of pigments. J. Cell. Biol.22, 443–451.

    Google Scholar 

  • Kuhn, H., 1970: Chemismus, Struktur und Entstehung der CarotinkristÄllchen in der Nebenkrone vonNarcissus poeticus L. var.“La Riante”. J. Ultrastruct. Res.33, 332–355.

    PubMed  Google Scholar 

  • Laborde, J. A., andA. R. Spurr, 1973: Chromoplast ultrastructure as affected by genes controlling grana retention and carotenoids in fruits ofCapsicum annuum. Amer. J. Bot.60, 736–744.

    Google Scholar 

  • Lance, A., 1958: Infrastructure des cellules du méristème apical et des jeunes ébauches foliaires deChrysanthemum segetum L. Ann. Sci. Nat. Bot. et Biol. Veg.19, 165–202.

    Google Scholar 

  • Lance-Nougarède, A., 1960: Développement inframicroscopique des chromoplastes au cours de l'ontogénèse des fleurs ligulées deChrysanthemum segetum. C. R. Acad. Sci. (Paris)250, 173–175.

    Google Scholar 

  • Laval-Martin, D., 1969: Evolution des pigments et des plastes au cours de la maturation de la tomate “cerise”. Bull. Soc. Franc. Physiol. Veg.15, 77–97.

    Google Scholar 

  • —,J. Quennemet etR. Monéger, 1972: Sur l'évolution des caroténoides et des chlorophylles du fruit de tomate “cerise” pendant sa croissance et sa maturation. C. R. Acad. Sci. (Paris)274, 2879–2882.

    Google Scholar 

  • Lefort-Tran, M., 1970: Discussion des techniques en vue de la conservation des pigments liposolubles en microscopie électronique. J. Micros.9, 881–890.

    Google Scholar 

  • Lehninger, A. L., andL. F. Remmert, 1959: An endogenous uncoupling and swelling agent in lines mitochondria and its enzymic formation. J. biol. Chem.234, 2459–2464.

    PubMed  Google Scholar 

  • Lichtenthaler, H. K., 1969: Die Plastoglobuli von Spinat, ihre Grö\e und Zusammensetzung wÄhrend der Chloroplastendegeneration. Protoplasma68, 315–326.

    Google Scholar 

  • —, 1970 a: Die Feinstruktur der Chromoplasten in plasmochromen Perigon-BlÄttern vonTulipa. Planta93, 143–151.

    Google Scholar 

  • —, 1970 b: Formation and function of plastoglobuli in plastids. 7‡ Cong. Intern. de Microsc. Elect. Grenoble3, 205–206.

    Google Scholar 

  • —, undM. Tevini, 1970: Die Verteilung der Pigmente, Plastidenchinone und Plastoglobuli auf verschiedene aus beschallten Spinatchloroplasten gewonnene Partikelfraktionen. Z. Pflanzenphysiol.62, 33–50.

    Google Scholar 

  • Marty, D., 1973: Aspects particuliers de l'ontogénèse des thylacoides dans les feuilles panachées deColeus blumei Benth. C. R. Acad. Sci. (Paris)277, 45–48.

    Google Scholar 

  • Mazliak, P., etA.-M. Catesson, 1968: Métabolisme des lipides dans le parenchyme externe de l'épiderme des pommes. II. Evolution de la lipogénèse au cours de la croissance des fruits, relation avec la morphologie des plastes. Fruits23, 247–257.

    Google Scholar 

  • Menke, W., 1963: Experiments made to elucidate the molecular structure of chloroplasts in photosynthetic mechanisms of green plants. Nat. Acad. Sci. natl. Res. Council Publ.1145, 537–544. Washington D.C.

    Google Scholar 

  • Meyer, A., 1883: Das Chlorophyllkorn in chemischer, morphologischer und biologischer Beziehung. Leipzig: Arthur Felix Verlag.

    Google Scholar 

  • Mitchell, P., 1966: Chemiosmotic coupling in oxydative and photosynthetic phosphorylation. Biol. Rev.41, 445–502.

    PubMed  Google Scholar 

  • Młodzianowski, F., andM. Kwintkiewicz, 1973: The inhibition of Kohlrabi chloroplast degeneration by kinetin. Protoplasma76, 211–226.

    PubMed  Google Scholar 

  • Monéger, R., 1968: Contribution à l'étude de l'influence exercée par la lumière sur la biosynthèse des caroténoÏdes chez laSpirodela polyrrhiza. Physiol. Veg.6, 367–402.

    Google Scholar 

  • Moor, H., 1964: Freeze-etching technique. J. appl. Phys.35, 3077.

    Google Scholar 

  • —, 1969: Freeze-etching. Int. Rev. Cytol.25, 391.

    PubMed  Google Scholar 

  • Murakami, S., 1964: Microstructure of chloroplast lamellae. J. Electron Microsc.13, 234–236.

    Google Scholar 

  • —, andP. S. Nobel, 1967: Lipids and light-dependent swelling of isolated spinach chloroplasts. Plant Cell Physiol.8, 657–671.

    Google Scholar 

  • Reynolds, E., 1963: The use of lead citrate at high pH as an electron-dense stain in electron microscopy. J. Cell Biol.17, 208–212.

    PubMed  Google Scholar 

  • Rosso, S. W., 1967: An ultrastructure study of the mature chromoplasts of the tangerine tomato (Lycopersicon esculentum var.“Golden jubilee”). J. Ultrastruct. Res.20, 179–189.

    PubMed  Google Scholar 

  • —, 1968: The ultrastructure of chromoplast development in red tomatoes. J. Ultrastruct. Res.25, 307–322.

    PubMed  Google Scholar 

  • Savelli, R., 1937: Sur la distribution des caroténoides rouges dans les chromoplastes. Protoplasma29, 601.

    Google Scholar 

  • Schimper, A. F. W., 1883: über die Entstehung der Chlorophyllkörner und Farbkörper. Bot. Z.41, 105–121, 137–153.

    Google Scholar 

  • Schurmann, P., undW. Villiger, 1967: VerÄnderungen des Chlorophyllgehaltes und der Chloroplastenfeinstruktur in den HochblÄttern vonDavidia involucrata Baill. Planta76, 335–347.

    Google Scholar 

  • Spurr, A. R., andW. M. Harris, 1968: Ultrastructure of chloroplasts and chromoplasts inCapsicum, annumm. I. Thylakoid membrane changes during fruit ripening. Amer. J. Bot.55, 1210–1224.

    Google Scholar 

  • Steffen, K., undG. Reck, 1964: Chromoplastenstudien. III. Die Chromoplastengenese und das Problem der Plastidenhüllen beiDaucus carota. Planta60, 627–648.

    Google Scholar 

  • Straus, W., 1956: Isolation of chromoplasts from carrots. Exp. Cell. Res.11, 289–296.

    PubMed  Google Scholar 

  • Thomson, W. W., 1966: Ultrastructure development of chromoplasts in “Valencia” oranges. Bot. Gaz.127, 133–139.

    Google Scholar 

  • —, 1967: The reversion of chromoplasts to chloroplasts in “Valencia” oranges. Cytologia32, 117–124.

    Google Scholar 

  • Toyama, S., 1967: Electron microscope studies on the morphogenesis of plastids. IV. Fine structure development of chromoplasts in the root ofDaucus carota. The Science Report, Tokyo Kyoiku Daigaku B12, 188, 245–253.

    Google Scholar 

  • Walles, B., 1971: Chromoplast development in a carotenoid mutant of Maize. Protoplasma73, 159–175.

    Google Scholar 

  • Weier, T. E., andA. A. Benson, 1966: The molecular nature of chloroplast membranes. In: Biochemistry of chloroplasts, Vol. I, pp. 91–113 (T. W. Goodwin, ed.). London-New York: Academic Press.

    Google Scholar 

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  1. Danielle Laval-Martin

    Present address: Laboratoire de Cytophysiologie de la Photosynthése, C. N. R. S., F-91190, Gif sur Yvette, France

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    Cet article constituera un chapitre de notre mémoire de Thèse d'état.

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    Laval-Martin, D. La maturation du fruit de tomate cerise: Mise en évidence, par cryodécapage, de l'évolution des chloroplastes en deux types de chromoplastes. Protoplasma 82, 33–59 (1974). https://doi.org/10.1007/BF01276870

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