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An ultrastructural and cytochemical investigation of the colonial green algaPediastrum tetras during zoospore formation

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Summary

Ultrastructural changes during zoospore formation and aggregation into motile, aggregating zoospores were examined in the colonial green algaPediastrum tetras. Developing zoospores are characterized by irregularly shaped nuclei, presence of peripheral networks of rough endoplasmic reticulum, chloroplasts with tightly apposed thylakoids and dictyosome cisternae which are compressed and reduced in size. A single membrane bound organelle with a fine granular matrix of moderate electron density of diameter ranging from 0.2 μm to 0.6 μm and associated with chloroplasts, mitochondria and endoplasmic reticulum was found only in adult cells. Although this organelle has the morphology of a microbody, it did not stain with 3,3′-diaminobenzidine (DAB) at pH 9.6 or pH 7.6, whereas mitochondrial membranes stained. No DAB staining was observed along the cell wall or the plasma membrane of zoospores, or associated with endoplasmic reticulum, plastid membranes or dictyosomes.

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  1. Adrienne A. Rogalski

    Present address: Department of Biological Sciences, University of Illinois, 60680, Chicago, IL, USA

Authors and Affiliations

  1. Whitman and Barnes Laboratories, Department of Biology, The University of Chicago, Chicago, Illinois, USA

    Adrienne A. Rogalski, J. Overton & M. Ruddat

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  1. Adrienne A. Rogalski
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  2. J. Overton
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  3. M. Ruddat
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Rogalski, A.A., Overton, J. & Ruddat, M. An ultrastructural and cytochemical investigation of the colonial green algaPediastrum tetras during zoospore formation. Protoplasma 91, 93–106 (1977). https://doi.org/10.1007/BF01276725

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  • DOI: https://doi.org/10.1007/BF01276725

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