Summary
Hydrolyzed walls (birefringent, Periodic acid/Schiff negative, remnants of primary walls that also lack polyuronides with free carboxyl groups) are demonstrated in the primary xylem of wheat and bean leaves. Walls with similar properties have been found in the primary xylem of a variety of tissues from different species, and are believed to be ubiquitous. It is shown that the pit membrane of intervessel pits between tracheary elements of willow is also a hydrolyzed wall. Combined with the observation byLiese (1965) it seems likely that the removal of non-cellulosic polysaccharides from primary walls unprotected by lignin is a general phenomenon that occurs late in the autolysis of all tracheary elements. Parenchyma cells that abut autolyzing tracheary elements appear to react to hydrolytic attack in a number of ways that are illustrated and discussed.
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References
Bailey, I. W., 1957: Die Struktur der Tüpfelmembranen bei den Tracheiden der Koniferen. Holz als Roh- und Werkstoff15, 210–213.
Cronshaw, J., andG. B. Bouck, 1965: The fine structure of differentiating xylem elements. J. Cell Biol.24, 415–431.
Esau, K., 1965: Plant anatomy, 2nd Edition. New York: John Wiley & Sons, Inc.
—, 1967: Minor veins inBeta leaves: structure related to function. Proc. Amer. Phil. Soc.111, 219–233.
Feder, N., andT. P. O'Brien, 1968: Plant microtechnique: some principles and new methods. Amer. J. Bot.55, 123–142.
Foster, R. C., 1967: Fine structure of tyloses in three species of theMyrtaccae. Aust. J. Bot.15, 25–34.
Gunning, B. E. S., J. S. Pate, andL. G. Briarty, 1968: Specialized “transfer cells” in minor veins of leaves and their possible significance in phloem translocation. J. Cell Biol.37, C 7-C 12.
Hepler, P. K., andE. H. Newcomb, 1964: Microtubules and fibrils in the cytoplasm ofColeus cells undergoing secondary wall deposition. J. Cell Biol.20, 529–533.
Liese, W., 1965: The fine structure of bordered pits in softwoods. In: “Cellular ultrastructure of woody plants” (W. A. Côté, Ed.) 271–290. Syracuse, N.Y.: Syracuse University Press.
Maser, M. D., T. P. O'Brien, andM. E. McCully, 1967: Shadowing of ultrathin sections of epoxy embedded tissues as an aid in three-dimensional interpretation. J. de Microscopie6, 305–312.
O'Brien, T. P., 1967: Observations on the fine structure of the oat coleoptile. I. The epidermal cells of the extreme apex. Protoplasma63, 385–416.
—,N. Feder, andM. E. McCully, 1964: Polychromatic staining of plant cell walls with toluidine blue O. Protoplasma59, 417–442.
—, andK. V. Thimann, 1967: Observations on the fine structure of the oat coleoptile. III. Correlated light and electron microscopy of the vascular tissues. Protoplasma63, 443–478.
Pickett-Heaps, J. D., 1966: Incorporation of radioactivity into wheat xylem walls. Planta71, 1–14.
—, 1967: The effects of colchicine on the ultrastructure of dividing plant cells, xylem wall differentiation and distribution of cytoplasmic microtubules. Develop. Biol.15, 206–236.
—, 1968: Further ultrastructural observations on polysaccharide localization in plant cells. J. Cell Sci.3, 55–64.
Rappay, G., andP. van Duijn, 1965: Chlorous acid as an agent for blocking tissue aldehydes. Stain Technol.40, 275–277
Robards, A. W., 1967: The xylem fibres ofSalix fragilis, L. J. Roy. Microscop. Soc.87, 329–352.
Schmid, R., 1965: The fine structure of pits in hardwoods. In: “Cellular ultrastructure of woody plants” (W. A. Côté, Ed.), 291–304. Syracuse, N.Y.: Syracuse University Press
Scurfield, G., 1967: Histochemistry of reaction wood differentiation inPinus radiata D. Don. Aust. J. Bot.18, 377–392.
Tsoumis, G., 1965: Light and electron microscopic evidence on the structure of the membrane of bordered pits in tracheids of conifers. In: “Cellular ultrastructure of woody plants” (W. A. Côté, Ed.), 305–317. Syracuse, N.Y.: Syracuse University Press.
Wardrop, A. B., 1964: The reaction anatomy of arborescent angiosperms. In: “Formation of Wood in Forest Trees” (M. H. Zimmermann, Ed.), 405–456. New York: Academic Press.
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O'Brien, T.P. Further observations on hydrolysis of the cell wall in the xylem. Protoplasma 69, 1–14 (1970). https://doi.org/10.1007/BF01276648
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DOI: https://doi.org/10.1007/BF01276648