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Contrasting effects of 5-hydroxytryptamine on the release of dopamine and acetylcholine in the nucleus accumbens of rat

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The interaction between 5-hydroxytryptamine (5-HT) and the release of dopamine and acetylcholine in the nucleus accumbens of rat was investigated. 5-HT (20μM) significantly decreased levels of [3H]-dopamine synthesized from [3H]-tyrosine. 5-HT also enhanced the release of [14C]-dopamine at all concentrations of K+ tested (up to 34 mM). The maximal effect of 5-HT occurred at 15 mM K+ and was reversed by methysergide (3μM). Increasing concentrations of Ca2+ facilitated the releasing effect of 5-HT up to 1.2 mM Ca2+. In contrast, 5-HT had an inhibitory effect on the K+-evoked release of [3H]-acetylcholine synthesized from [3H]-choline. 5-HT was not effective at non-depolarizing concentrations of K+. Increasing the external Ca2+ concentration to 2.5 mM overcame the inhibition of [3H]-acetylcholine release due to 5-HT. These results indicate that 5-HT has at least two actions in the nucleus accumbens, one which is a methysergidesensitive facilitatory action on dopamine release promoted by Ca2+ entry and a second, inhibitory effect on the K+-evoked release of acetylcholine, which is not blocked by methysergide and appears to operate by reducing Ca2+ influx.

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de Belleroche, J., Gardiner, I.M. Contrasting effects of 5-hydroxytryptamine on the release of dopamine and acetylcholine in the nucleus accumbens of rat. J. Neural Transmission 55, 227–242 (1982). https://doi.org/10.1007/BF01276578

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