Summary
Experiments were carried out in order to localize products of diaminobenzidine in illuminated and non-illuminated isolated pea chloroplasts. The number of particles exposed by deepetching on the endoplasmic surface (ES) of the thylakoid membrane in illuminated chloroplasts was found to be higher than that of non-illuminated ones. A similar result was obtained for protrusions counted on the fractured faces of the corresponding half of the thylakoid membrane (EF). It is suggested that both the particles and the corresponding protrusions are deposits of oxidized diaminobenzidine formed in the light. These results are in agreement with biochemical experiments, indicating that diaminobenzidine donates electrons on the endoplasmic surface (ES) of the thylakoid.
Similar content being viewed by others
References
Arnon, D. I., 1949: Copper enzymes in isolated chloroplasts polyphenoloxidase inBeta vulgaris. Plant Physiol.24, 1–15.
Ben-Hayyim, G., Z. Drechsler, andJ. Neumann, 1974: Evidence for sideness of the thylakoid membrane: Studying photophosphorylation by photosystem I. Proc. 3rd Int. Congr. on photosynthesis (M. Avron, ed.), pp. 873–881. Israel: Rehovot.
Branton, D., S. Bullivand, N. B. Gilula, N. J. Karnovsky, H. Moor, K. Mühlethaler, D. H. Northcote, L. Packer, B. Satir, P. Satir, V. Speth, L. A. Strachlin, R. L. Steeve, andR. S. Weinstein, 1975: Freeze-etching nomenclature. Science190, 54–56.
Fahimi, H. D., 1969: Cytochemical localization of peroxidatic activity of catalase in rat hepatic microbodies (Peroxisomes). J. Cell. Biol.43, 275–288.
Frederick, S. E., andE. H. Newcomb, 1969: Cytochemical localization of catalase in leaf microbodies (Peroxisomes). J. Cell. Biol.43, 343–353.
Goffer, J., andJ. Neumann, 1973: Evidence for system I mediated non-cyclic photophosphorylation in chloroplasts. FEBS Letters36, 61–64.
Goldfischer, S., andE. Essner, 1969: Further observations on the peroxidatic activities of microbodies (Peroxisomes). J. Histochem. Cytochem.17, 681–685.
Goodenough, U. W., andL. A. Staehelin, 1971: Structural differentiation of stacked and unstacked chloroplast membranes. Freeze-etch electron microscopy of wild-type and mutant strains ofChlamydomonas. J. Cell. Biol.48, 594–619.
Graham, R. C., andM. J. Karnovsky, 1966: The early stages of adsorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: Ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem.14, 291–302.
Karnovsky, M. J., 1967: The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J. Cell. Biol.35, 213–236.
Moor, H., andK. Mühlethaler, 1963: Fine structure in frozen-etched yeast cells. J. Cell. Biol.17, 609–628.
Nam-Hai, C., 1972: Photooxidation of 3,3′ diaminobenzidine by blue-green algae inChlamydomonas reinhardii. Biochim. biophys. Acta267, 179–189.
Nir, I., andD. C. Pease, 1973: Chloroplast organization and the ultrastructural localization of photosystem I and II. J. Ultrastruct. Res.42, 534–550.
—, andA. M. Seligman, 1970: Photooxidation of diaminobenzidine by chloroplast lamellae. J. Cell. Biol.46, 617–620.
Novikoff, A. B., andS. Goldfischer, 1969: Visualization of peroxisomes (microbodies) and mitochondria with diaminobenzidine. J. Histochem. Cytochem.17 675–680.
Seligman, A. M., M. J. Karnovsky, H. L. Wasserkrug, andJ. C. Hanker, 1968: Non-droplet ultrastructural demonstration of cytochrome oxidase activity with a polymerizing osmiophilic reagent diaminobenzidine. J. Cell. Biol.38, 1–14.
Trebst, A., 1974: Energy conservation in photosynthetic electron transport of chloroplasts. Ann. Rev. Plant Physiol.25, 423–458.
Vigil, E. L., 1969: Intracellular localization of catalase (peroxidatic) activity in plant microbodies. J. Histochem. Cytochem.17, 425–428.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Porat, N., Ben-Hayyim, G. & Friedberg, I. Localization of oxidized 3,3′-diaminobenzidine deposits in chloroplasts. Protoplasma 93, 397–403 (1978). https://doi.org/10.1007/BF01276553
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01276553