In plant cell suspension cultures sensitive to the herbicide amiprophos-methyl (APM), 1 to 3 μM APM completely depolymerized both cortical and mitotic microtubule (MT) arrays in 1 hour. In comparison, a 2 hour application of 3 mM colchicine had no effect on MT arrays. Recovery from APM treatment occurred as early as 5 minutes after removal of APM. Short, cortical MTs were visible in 3 hours and complete MT arrays were found within 22 hours after drug removal.
Sensitivity to APM-induced MT depolymerization varied according to species but was increased or decreased by varying the mitotic rate in cultures. The results indicated APM sensitivity was related to lowered stability of MT arrays in rapidly cycling cells. APM treatment may help distinguish “stabilized” cortical MTs in elongating cells and “nonstabilized” cortical MTs in rapidly dividing cells.
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Falconer, M.M., Seagull, R.W. Amiprophos-methyl (APM): A rapid, reversible, anti-microtuble agent for plant cell cultures. Protoplasma 136, 118–124 (1987). https://doi.org/10.1007/BF01276360
- Amiprophos-methyl (APM)
- Microtubule inhibitor
- Plant cell cultures