Skip to main content
SpringerLink
Log in

Morphology of the nucleo-cytoplasmic interactions during the development ofAcetabularia cells

I. The vegetative phase

  • Original Papers
  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

The ultrastructure of the growing and maturing primary nucleus ofAcetabularia mediterranea andAcetabularia major has been studied with the use of various fixation procedures. Particular interest has been focused on the details of the nuclear periphery and the perinuclear region. It is demonstrated that early in nuclear growth a characteristic perinuclear structural complex is formed which is, among the eukaryotic cells, unique toAcetabularia and related genera. This perinuclear system consists essentially of

  1. a)

    the nuclear envelope with a very high pore frequency and various pore complex associations with granular and/or threadlike structures some of which are continuous with the nucleolus;

  2. b)

    an approximately 100 nm thick intermediate zone densely filled with a filamentous material and occasional small membraneous structures from which the typical cytoplasmic and nuclear organelles and particles are excluded;

  3. c)

    an adjacent lacunar labyrinthum which is interrupted by many plasmatic junction channels between the intermediate zone and the free cytoplasm;

  4. d)

    numerous dense perinuclear bodies in the juxtanuclear cytoplasm which are especially frequent at the junction channels and reveal a composition of aggregated fibrillar and granular structures;

  5. e)

    very dense exclusively fibrillar aggregates which occur either in association with the perinuclear region of the lacunar labyrinthum or, somewhat further out, in the cytoplasmic strands between the branches of the lacunar labyrinthum in the form of slender, characteristic rods or “sausages”.

A variety of other membraneous and non-membraneous structures characteristic of the juxtanuclear cytoplasm is described. The organization of the individual components in this special complex is summarized in a model drawing. The dynamic and transitory nature of this perinuclear complex apparatus is emphasized and its possible role in nuclear functions and in regulating nucleocytoplasmic interactions is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Allen, E. R., andM. D. Cave, 1969: Cytochemical and ultrastructural studies of ribonucleoprotein containing structures in oocytes ofAcheta domesticus. Z. Zellforsch.101, 63–71.

    Google Scholar 

  • Beermann, W., 1964: Control of differentiation at the chromosomal level. J. exp. Zool.157, 49–61.

    Google Scholar 

  • Behnke, O., 1968: An electron microscope study of the megacaryocyte of the rat bone marrow. I. The development of the demarcation membrane system and the platelet surface coat. J. Ultrastruct. Res.24, 412–433.

    Google Scholar 

  • Berger, S., W.Herth, W. W.Franke, H.Falk, H.Spring, and H. G.Schweiger, 1974: Morphology of the nucleo-cytoplasmic interaction during the development ofAcetabularia cells. II. The generative phase. In preparation.

  • Bernhard, W., 1969: A new staining procedure for electron microscopical cytology. J. Ultrastruct. Res.27, 250–265.

    Google Scholar 

  • Beth, K., 1953: Experimentelle Untersuchungen über die Wirkung des Lichtes auf die Formbildung von kernhaltigen und kernlosenAcetabularia-Zellen. Z. Naturforsch.8 b, 334–342.

    Google Scholar 

  • Boloukhère-Presburg, M., 1969: Ultrastructure de l'algueAcetabularia mediterranea au cours du cycle biologique et dans differentes conditions experimentales. Thesis, Université Libre de Bruxelles.

  • Boloukhère, M., 1970: Ultrastructure ofAcetabularia mediterranea in the course of formation of the secondary nuclei. In: Biology ofAcetabularia, pp. 145–175. (J. Brachet andS. Bonotto, eds.). New York: Academic Press.

    Google Scholar 

  • Brändle, E., undK. Zetsche, 1973: Zur Lokalisation der α-Amanitin sensitiven RNA-Polymerase in Zellkernen vonAcetabularia. Planta111, 209–217.

    Google Scholar 

  • Burr, F. A., andJ. A. West, 1971: Comparative ultrastructure of the primary nucleus inBryopsis andAcetabularia. J. Phycol.7, 103–113.

    Google Scholar 

  • Busch, H., andK. Smetana, 1970: The nucleolus. New York: Academic Press.

    Google Scholar 

  • Callan, H. C., andL. Lloyd, 1960: Lampbrush chromosomes of crested newtsTriturus cristatus. Phil. Trans. Roy. Soc. (London)243 B, 135–219.

    Google Scholar 

  • Clérot, J.-C., 1968: Mise en évidence par cytochimie ultrastructurale de l'émission de protéines par le noyau d'auxocytes de batraciens. J. Microscopie7, 973–992.

    Google Scholar 

  • Coggeshall, R. E., andD. W. Fawcett, 1964: The fine structure of the central nervous system of the leech,Hirudo medicinalis. J. Neurophysiol.27, 229–289.

    Google Scholar 

  • Comes, P., andW. W. Franke, 1970: Composition, structure and function of HeLa cell nuclear envelope. I. Structural data. Z. Zellforsch.107, 240–248.

    Google Scholar 

  • Conway, C. M., 1971: Evidence for RNA in the heavy bodies of sea urchin eggs. J. Cell Biol.51, 889–893.

    Google Scholar 

  • —, andC. B. Metz, 1970: Cytochemical demonstrations of RNA in heavy bodies of sea urchin eggs. J. Cell Biol.47, 40 a.

    Google Scholar 

  • Crawley, J. C. W., 1963: The fine structure ofAcetabularia mediterranea. Exp. Cell Res.32, 368–378.

    Google Scholar 

  • —, 1965: The fine structure of isolatedAcetabularia nuclei. Planta65, 205–217.

    Google Scholar 

  • Daniels, E. W., andE. P. Breyer, 1967: Ultrastructure of the giant amoebaPelomyxa palustris. J. Protozool.14, 167–179.

    Google Scholar 

  • —,J. M. McNiff, andD. R. Ekberg, 1969: Nucleopores of the giant amoeba,Pelomyxa carolinensis. Z. Zellforsch.98, 357–363.

    Google Scholar 

  • Dhainaut, A., 1970: Étude en microscopie électronique et par autoradiographie a haute résolution des extrusions nucléaires au cours de l'ovogenèse deNereis pelagica. J. Microscopie9, 99–118.

    Google Scholar 

  • Eddy, E. M., andS. Ito, 1971: Fine structural and radioautographic observations on dense perinuclear cytoplasmic material in tadpole oocytes. J. Cell Biol.49, 90–108.

    Google Scholar 

  • Engelhardt, P., andK. Pusa, 1972: Nuclear pore complexes: “press-stud” elements of chromomes in pairing and control. Nature New Biol.240, 163–166.

    Google Scholar 

  • Fabergé, A. C., 1973: Direct demonstration of eight-fold symmetry in nuclear pores. Z. Zellforsch.136, 183–190.

    Google Scholar 

  • Fawcett, D. W., 1966: On the occurence of a fibrous lamina on the inner aspect of the nuclear envelope in certain cells of vertebrates. Amer. J. Anat.119, 129–145.

    Google Scholar 

  • -Fawcett, D. W., 1972: Observations on cell differentiation and organelle continuity in spermatogenesis. In: The genetics of the spermatozoon, pp. 37–68. (R. A.Beatty) and S.Gluecksohn-Waelsch, eds.). Edinburgh.

  • Feldherr, C. M., 1972: Structure and function of the nuclear envelope. Advances in cell and molecular biology, Vol.2, 273–307.

    Google Scholar 

  • Flickinger, C. J., 1970: The fine structure of the nuclear envelope in amebae: alterations following nuclear transplantation. Exp. Cell Res.60, 225–236.

    Google Scholar 

  • Franke, W. W., 1966: Isolated nuclear membranes. J. Cell Biol.31, 619–623.

    Google Scholar 

  • —, 1967: Zur Feinstruktur isolierter Kernmembranen aus tierischen Zellen. Z. Zellforsch.80, 585–593.

    Google Scholar 

  • —, 1970: On the universality of nuclear pore complex structure. Z. Zellforsch.105, 405–429.

    Google Scholar 

  • - 1974: Structures, functions and biochemistry of the nuclear envelope. Int. Rev. Cytol. In press.

  • —,W. A. Eckert, andS. Krien, 1971 b: Cytomembrane differentiation in a ciliate,Tetrahymena pyriformis. I. Endoplasmic reticulum and dictyosomal equivalents. Z. Zellforsch.119, 577–604.

    Google Scholar 

  • —, andH. Falk, 1970: Appearance of nuclear pore complexes after Bernhard's staining procedure. Histochemie24, 266–278.

    Google Scholar 

  • —,J. Kartenbeck, S. Krien, W. J. Vanderwoude, U. Scheer, andD. J. Morré, 1972: Inter- and intracisternal elements of the Golgi apparatus. Z. Zellforsch.132, 365–380.

    Google Scholar 

  • — —,H. Zentgraf, U. Scheer, andH. Falk, 1971 a: Membrane-to-membrane cross-bridges. J. Cell Biol.51, 881–888.

    Google Scholar 

  • —,S. Krien, andR. M. Brown, 1969: Simultaneous glutaraldehyde-osmium tetroxide fixation with postosmication. Histochemie19, 162–164.

    Google Scholar 

  • Franke, W. W., andU. Scheer, 1970 a: The ultrastructure of the nuclear envelope of amphibian oocytes: a re-investigation. I. The mature oocyte. J. Ultrastruct. Res.30, 288–316.

    Google Scholar 

  • — —, 1970 b: The ultrastructure of the nuclear envelope of amphibian oocytes: a reinvestigation. II. The immature oocyte and dynamic aspects. J. Ultrastruct. Res.30, 317–327.

    Google Scholar 

  • — —, 1971: Some structural differentiations in the HeLa cell: heavy bodies, annulate lamellae, and cotte de maillet endoplasmic reticulum. Cytobiologie4, 317–329.

    Google Scholar 

  • — —, 1972: Structural details of dictyosomal pores. J. Ultrastruct. Res.40, 132–144.

    Google Scholar 

  • — —, 1974: Structures and functions of the nuclear envelope. In: The cell nucleus, Vol. 1 (H. Busch, ed.). New York: Academic Press. In press.

    Google Scholar 

  • Friederici, H. H. R., 1969: On the diaphragm across fenestrae of capillary endothelium. J. Ultrastruct. Res.27, 373–375.

    Google Scholar 

  • Gall, J. G., 1964: Electron microscopy of the nuclear envelope. In: Protoplasmatologia, pp. 4–25. Vol. V/2 (M. Alfert, H. Bauer, andC. V. Harding, eds.). Wien: Springer-Verlag.

    Google Scholar 

  • —, 1967: Octogonal nuclear pores. J. Cell Biol.32, 391–399.

    Google Scholar 

  • Gerin, Y., 1971: Étude par cytochimie ultrastructurale des corpuscules périnucléaires présents dans les jeunes oocytes deIlyanassa obsoleta Say (Mollusca gastéropode). J. embryol. exp. Morph.25, 423–438.

    Google Scholar 

  • Gouranton, J., 1969: L'enveloppe nucléaire. Ann. Biol.8, 385–409.

    Google Scholar 

  • Gray, E. G., andGuillery, R. W., 1963: On nuclear structure in the ventral nerve cord of the leechHirudo medicinalis. Z. Zellforsch.59, 738–745.

    Google Scholar 

  • Greider, M. H., W. J. Kostir, andW. J. Frajola, 1956: Electron microscopy of the nuclear membrane ofAmoeba proteus. J. biophys. biochem. Cytol.2, Suppl., 445–447.

    Google Scholar 

  • Hämmerling, J., 1931: Entwicklung und Formbildungsvermögen vonAcetabularia mediterranea. I. Die normale Entwicklung. Biol. Zentralbl.51, 633–647.

    Google Scholar 

  • —, 1944: Zur Lebensweise, Fortpflanzung und Entwicklung verschiedenerDasycladaceen. Arch. Protistenk.97, 7–56.

    Google Scholar 

  • —, 1957: Nucleus and cytoplasm inAcetabularia. VIIIe Congrès International de Botanique Paris 1957. Comp. Rend. des Séances et Rapports et Communications deposés lors du Congrès dans la Section10, 87–103.

    Google Scholar 

  • —, 1963: Nucleo-cytoplasmic interactions inAcetabularia and other cells. Ann. Rev. Plant Physiol.14, 65–92.

    Google Scholar 

  • —,H. Clauss, K. Keck, G. Richter, andG. Werz, 1958: Growth and protein synthesis in nucleated and enucleated cells. Exp. Cell Res. Suppl.6, 210–226.

    Google Scholar 

  • Harris, P., 1967: Structural changes following fertilization in the sea urchin egg. Exp. Cell Res.48, 569–581.

    Google Scholar 

  • —, andT. W. James, 1952: Electron microscopical observations on the nuclei ofAmoeba proteus. Experientia8, 384–385.

    Google Scholar 

  • Jaworska, H., S. Avanzi, andA. Lima-de-Faria, 1973: Amplification of ribosomal DNA inAcheta. VIII. Binding of H3-Actinomycin to DNA in the nucleus and cytoplasm. Hereditas74, 205–210.

    Google Scholar 

  • —, andA. Lima-de-Faria, 1973: Amplification of ribosomal DNA inAcheta. VI. Ultrastructure of two types of nucleolar components associated with ribosomal DNA. Hereditas74, 169–186.

    Google Scholar 

  • — —, 1973: Amplification of ribosomal DNA inAcheta. VII. Transfer of DNA-RNA assemblies from the nucleus to the cytoplasm. Hereditas74, 187–204.

    Google Scholar 

  • Kartenbeck, J., H. Zentgraf, U. Scheer, andW. W. Franke, 1971: The nuclear envelope in freeze-etching. Erg. Anat. Entwicklungsgeschichte45, 1–55.

    Google Scholar 

  • Kay, R. R., andJ. R. Johnston, 1973: The nuclear envelope: Current problems of structure and of function. Sub-Cell. Biochem.2, 127–167.

    Google Scholar 

  • Kessel, R. G., 1968: Annulate lamellae. J. Ultrastruct. Res. Suppl.10, 1–82.

    Google Scholar 

  • —, 1969: Fine structure of the pore-annulus complex in the nuclear envelope and annulate lamellae of germ cells. Z. Zellforsch.94, 441–453.

    Google Scholar 

  • —, 1973: Structure and function of the nuclear envelope and related cytomembranes. Progress in surface and membrane science, Vol.6, 243–329.

    Google Scholar 

  • Kezer, J., H. C. Macgregor, andE. Schabtach, 1971: Observations on the membranous components of amphibian oocyte nucleoli. J. Cell Sci.8, 1–17.

    Google Scholar 

  • Kiermayer, O., 1971: Elektronenmikroskopischer Nachweis cytoplasmatischer Vesikel beiMicrasterias denticulata Bréb. Planta96, 74–80.

    Google Scholar 

  • La Cour, L. F., andB. Wells, 1972: The nuclear pores of early meiotic prophase nuclei of plants. Z. Zellforsch.123, 178–194.

    Google Scholar 

  • Lane, N. J., 1967: Spheroidal and ring nucleoli in amphibian oocytes. J. Cell Biol.35, 421–434.

    Google Scholar 

  • Mahowald, A. P., 1971: Origin and continuity of polar granules. Results and problems in cell differentiation, Vol.2, 158–169.

    Google Scholar 

  • Marchant, H. J., andJ. D. Pickett-Heaps, 1970: Ultrastructure and differentiation ofHydrodictyon reticulatum. I. Mitosis in the coenobium. Aust. J. Biol. Sci.23, 1173–1186.

    Google Scholar 

  • Maul, G., 1971: Structure and formation of pores in fenestrated capillaries. J. Ultrastruct. Res.36, 768–782.

    Google Scholar 

  • Maul, G. G., J. W. Price, andM. W. Lieberman, 1971: Formation and distribution of nuclear pore complexes in interphase. J. Cell Biol.51, 405–418.

    Google Scholar 

  • —,H. M. Maul, J. E. Scogna, M. W. Lieberman, G. S. Stein, B. Y. L. Hsu, andT. W. Borun, 1972: Time sequence of nuclear pore formation in phytohemagglutinin-stimulated lymphocytes and in HeLa cells during the cell cycle. J. Cell Biol.55, 433–447.

    Google Scholar 

  • Maul, H. M., B. Y. L. Hsu, T. M. Borun, andG. G. Maul, 1973: Effect of metabolic inhibitors on nuclear pore formation during the HeLa S3 cell cycle. J. Cell Biol.59, 669–676.

    Google Scholar 

  • McDonald, K., 1972: The ultrastructure of mitosis in the marine red algaMembranoptera platyphylla. J. Phycol.8, 156–166.

    Google Scholar 

  • Mercer, E. H., 1959: An electron microscopic study ofAmoeba proteus. Proc. Roy. Soc. B150, 216–232.

    Google Scholar 

  • Merriam, R. W., 1961: Nuclear envelope structure during cell division inChaetopterus eggs. Exp. Cell Res.22, 93–107.

    Google Scholar 

  • —, 1962: Some dynamic aspects of the nuclear envelope. J. Cell Biol.12, 79–90.

    Google Scholar 

  • Miller, O. L., 1966: Structure and composition of peripheral nucleoli of salamander oocytes. Nat. Cancer Inst. Monograph23, 53–66.

    Google Scholar 

  • —, andB. R. Beatty, 1969: Visualization of nucleolar genes. Science164, 955–957.

    Google Scholar 

  • Monneron, A., andW. Bernhard, 1969: Fine structural organization of the interphase nucleus in some mammalian cells. J. Ultrastruct. Res.27, 266–288.

    Google Scholar 

  • Palade, G. E., andR. R. Bruns, 1968: Structural modulations of plasmalemmal vesicles. J. Cell Biol.37, 633–649.

    Google Scholar 

  • Pappas, G. D., 1956: The fine structure of the nuclear envelope ofAmoeba proteus. J. biophys. biochem. Cytol. Suppl.2, 431–434.

    Google Scholar 

  • Pickett-Heaps, J. D., 1972: Cell division inTetraedron. Ann. Bot.36, 693–701.

    Google Scholar 

  • Rasmussen, S. W., 1973: Ultrastructural studies of spermatogenesis inDrosophila melanogaster Meigen. Z. Zellforsch.140, 125–144.

    Google Scholar 

  • Reynolds, E. S., 1963: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol.17, 208–211.

    Google Scholar 

  • Roberts, K., andD. H. Northcote, 1970: Structure of the nuclear pore in higher plants. Nature228, 385–386.

    Google Scholar 

  • Roberts, K., andD. H. Northcote, 1971: Ultrastructure of the nuclear envelope; structural aspects of the interphase nucleus of sycamore suspension culture cells. Microsc. Acta71, 102–120.

    Google Scholar 

  • Scheer, U., 1970: Strukturen und Funktionen der Porenkomplexe in der Amphibieneizelle. Thesis, Universität Freiburg, pp. 1–174.

  • —, 1973: Nuclear pore flow rate of ribosomal RNA and chain growth rate of its precursor during oogenesis ofXenopus laevis. Develop. Biol.30, 13–28.

    Google Scholar 

  • —, andW. W. Franke, 1972: Annulate lamellae in plant cells: formation during microsporogenesis and pollen development inCanna generalis Bailey. Planta107, 145–159.

    Google Scholar 

  • —,M. F. Trendelenburg, andW. W. Franke, 1973: Transcription of ribosomal RNA cistrons. Correlation of morphological and biochemical data. Exp. Cell Res.80, 175–190.

    Google Scholar 

  • Schjeide, O. A., F. Galey, E. A. Grellert, R. I-San Lin, J. de Vellis, andJ. F. Mead, 1970: Macromolecules in oocyte maturation. Biol. Reprod.2, 14–43.

    Google Scholar 

  • Schweiger, H. G., 1969: Cell biology ofAcetabularia. Curr. Top. Microbiol. Immunol.50, 1–36.

    Google Scholar 

  • -Schweiger, H. G., S.Berger, K.Kloppstech, K.Apel, and M.Schweiger, 1974: Some fine structural and biochemical features ofAcetabularia major (Chlorophyta, Dasycladaceae) grown in the laboratory. Phycologia13. In press.

  • Simard, R., F. Sakr, andJ.-P. Bachellerie, 1973: Ribosomal precursor particles in ascites tumor cell nucleoli. Exp. Cell Res.81, 1–7.

    Google Scholar 

  • Speth, V., andF. Wunderlich, 1970: The macronuclear envelope ofTetrahymena pyriformis GL in different physiological stages. III. Appearance of freeze-etched nuclear pore complexes. J. Cell Biol.47, 772–777.

    Google Scholar 

  • Spring, H., H.Falk, W. W.Franke, and S.Berger, 1974: Morphology of the nucleocytoplasmic interaction during the development ofAcetabularia cells. III. The cytochemistry of the perinuclear bodies. In preparation.

  • Stelly, N., B. J. Stevens etJ. André, 1970: Étude cytochimique de la lamelle dense de l'enveloppe nucléaire. J. Microscopie9, 1015–1028.

    Google Scholar 

  • Stevens, A. R., 1967: Machinery for exchange across the nuclear envelope. In: The control of nuclear activity, pp. 189–271. (L.Goldstein, ed.). New Jersey: Englewood Cliffs.

  • Stevens, B. J., andJ. André, 1969: The nuclear envelope. In: Handbook of molecular cytology, pp. 837–871. (A. Lima-de-Faria, ed.). Amsterdam-London: North Holland Publishing Comp.

    Google Scholar 

  • —, andH. Swift, 1966: RNA transport from nucleus to cytoplasm inChironomus salivary glands. J. Cell Biol.31, 55–77.

    Google Scholar 

  • Taylor, G. T., andE. Anderson, 1969: Cytochemical and fine structural analysis of oogenesis in the gastropodIlyanassa obsoleta. J. Morph.129, 211–248.

    Google Scholar 

  • Thair, B. W., andA. B. Wardrop, 1971: The structure and arrangement of nuclear pores in plant cells. Planta100, 1–17.

    Google Scholar 

  • Trendelenburg, M. F., U.Scheer, and W. W.Franke, 1974: Effect of Actinomycin D on the template association of nascent pre-rRNP in amphibian oocyte nucleoli. In preparation.

  • Van Gansen, P., etM. Boloukhère-Presburg, 1965: Ultrastructure de l'algue unicellulaireAcetabularia mediterranea Lmx (chloroplasts, ribosomes et noyeau). J. Microscopie4, 347–362.

    Google Scholar 

  • Vazquez-Nin, G., andW. Bernhard, 1971: Comparative ultrastructural study of perichromatin—and Balbiani ring granules. J. Ultrastruct. Res.36, 842–860.

    Google Scholar 

  • Vivier, E., 1967: Observations ultrastructurales sur l'enveloppe nucléaire et ses “pores” chez des Sporozoaires. J. Microscopie6, 371–390.

    Google Scholar 

  • Watson, M. L., 1959: Further observations on the nuclear envelope of animal cell. J. biophys. biochem. Cytol.6, 147–155.

    Google Scholar 

  • —, 1962: Observations on a granule associated with chromatin in the nuclei of cells of rat and mouse. J. Cell Biol.13, 162–167.

    Google Scholar 

  • Weakley, B. S., 1971: Basic protein and RNA in the cytoplasm of the ovarian oocyte in the golden hamster. Z. Zellforsch.112, 69–84.

    Google Scholar 

  • Werz, G., 1962: Zur Frage der Elimination von Ribosenucleinsäure und Protein aus dem Zellkern vonAcetabularia mediterranea. Planta57, 636–655.

    Google Scholar 

  • —, 1964: Untersuchungen zur Feinstruktur des Zellkernes und des perinukleären Plasmas vonAcetabularia. Planta62, 255–271.

    Google Scholar 

  • Wiener, J., D. Spiro, andW. R. Loewenstein, 1965: Ultrastructure and permeability of nuclear membranes. J. Cell Biol.27, 107–117.

    Google Scholar 

  • Wischnitzer, S., 1970: The annulate lamellae. Int. Rev. Cytol.27, 65–100.

    Google Scholar 

  • —, 1973: The submicroscopic morphology of the interphase nucleus. Int. Rev. Cytol.34, 1–48.

    Google Scholar 

  • Woodcock, C. L. F., andG. J. Miller, 1973: Ultrastructural features of the life cycle ofAcetabularia mediterranea. II. Events associated with the division of the primary nucleus and the formation of cysts. Protoplasma77, 331–341.

    Google Scholar 

  • Wunderlich, F., andW. W. Franke, 1968: Structure of macronuclear envelopes ofTetrahymena pyriformis in the stationary phase of growth. J. Cell Biol.38, 458–462.

    Google Scholar 

  • —, andV. Speth, 1972: The macronuclear envelope ofTetrahymena pyriformis GL in different physiological stages. IV. Structural and functional aspect of nuclear pore complexes. J. Microscopie13, 361–382.

    Google Scholar 

  • Yoo, B. Y., andS. T. Bayley, 1967: The structure of pores in isolated pea nuclei. J. Ultrastruct. Res.18, 651–660.

    Google Scholar 

  • Zerban, H., M. Wehner undG. Werz, 1973: Über die Feinstruktur des Zellkerns vonAcetabularia nach Gefrierätzung. Planta114, 239–250.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institut für experimentelle Pathologie, Abteilung für Membranbiologie und Biochemie, Deutsches Krebsforschungszentrum, D-6900, Heidelberg, Germany

    W. W. Franke, H. Spring, U. Scheer & M. F. Trendelenburg

  2. Max-Planck-Institut für Zellbiologie, D-2940, Wilhelmshaven, Germany

    S. Berger & H. G. Schweiger

  3. Institut für Biologie II, Abteilung für Zellbiologie, Universität Freiburg i. Br., D-7800, Freiburg i. Br., Germany

    H. Falk & W. Herth

Authors
  1. W. W. Franke
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Berger
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Falk
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. Spring
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. U. Scheer
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. W. Herth
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. M. F. Trendelenburg
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. H. G. Schweiger
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Franke, W.W., Berger, S., Falk, H. et al. Morphology of the nucleo-cytoplasmic interactions during the development ofAcetabularia cells. Protoplasma 82, 249–282 (1974). https://doi.org/10.1007/BF01276311

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01276311

Keywords

Search

Navigation