Summary
Ultrastructural study of a dikaryon of the basidiomyceteSchizophyllum commune showed that treatment with griseofulvin affected the site of the dividing nuclei and the location and structure of the septa. The microtubules were considered to be the primary target of griseofulvin, since they participate in nuclear division and movement in the hyphae, and their assembly is known to be in other organisms than fungi inhibited by griseofulvin. It is pointed out that dikaryotic hyphae with two nuclei and a clamp connection per cell are more sensitive indicators of the effect of griseofulvin than homokaryotic hyphae, whose structure is less complex.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Giesy, R. M., Day, P. R., 1965: The septal pores ofCoprinus lagopus in relation to nuclear migration. Amer. J. Bot.52, 287–293.
Girbardt, M., 1968: Ultrastructure and dynamics of the moving nucleus. In: Aspects of cell motility. 22. Symp. Soc. Exp. Biol. Oxford (Miller, P. L., ed.), pp. 249–259. Cambridge: University Press.
—, 1978: Historical review and introduction. In: Nuclear division in fungi (Heath, B. I., ed.), pp. 1–20. New York-San Francisco-London: Academic Press.
—, 1979: A microfilamentous septal belt (FSB) during induction of cytokinesis inTrametes versicolor (L. ex Fr.). Exp. Mycology3, 215–228.
Malavista, S. E., Sato, H., Creasey, A. W., 1976: Dissociation of the mitotic spindle in oocytes to griseofulvin and vinblastine. Exp. Cell Res.99, 193–197.
Niederpruem, D. J., 1969: Direct studies of nuclear movements inSchizophyllum commune. Arch. Mikrobiol.64, 387–395.
North, J., 1977: The effect of griseofulvin on diploid strains ofCoprinus lagopus. J. gen. Microbiol.98, 529–534.
Raper, J. R., 1966: Genetics of sexuality in higher fungi. New York: Ronald Press.
—,Raudaskoski, M., 1968: Secondary mutations at the β incompatibility locus ofSchizophyllum. Heredity23, 109–117.
Raudaskoski, M., 1970: Occurrence of microtubules and microfilaments, and origin of septa in dikaryotic hyphae ofSchizophyllum commune. Protoplasma70, 415–422.
—, 1972: Secondary mutations at the β incompatibility locus and nuclear migration in the basidiomyceteSchizophyllum commune. Hereditas72, 175–182.
—,Huttunen, E., 1977: The effect of griseofulvin on nuclear distribution in a dikaryon ofSchizophyllum commune. Mykosen20, 339–348.
Roobol, A., Gull, K., Pogson, C. I., 1976: Inhibition by griseofulvin of microtubule assemblyin vitro. FEBS letters67, 248–251.
— 1977: Griseofulvin-induced aggregation of microtubule protein. Biochem. J.167, 39–43.
Valla, G., 1979: Effects of griseofulvin on cytology, growth, mitosis and branching ofPolyporus arcularius. Trans. Br. mycol. Soc.73, 135–139.
Weber, K., Wehland, J., Herzog, W., 1976: Griseofulvin interacts with microtubules bothin vivo andin vitro. J. mol. Biol.102, 817–829.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Raudaskoski, M. Griseofulvin-induced alterations in site of dividing nuclei and structure of septa in a dikaryon ofSchizophyllum commune . Protoplasma 103, 323–331 (1980). https://doi.org/10.1007/BF01276277
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01276277