Summary
A hyperdiploid aneuploid karyotype, consisting of 7 chromosomes, has been found in a tumorous suspension cell culture ofCrepis capillaris (2 n=6). Giemsa C-banding has revealed that these 7 chromosomes show consistent patterns of differential staining in all dividing cells. This stable karyotypic situation has persisted during 18 months of cytological monitoring of the culture. Comparison with the diploid C-banded complement of the root tip indicates that numerous structural rearrangements must have occurred during the formation of the aneuploid complement. A likely pathway for evolution of this karyotype involves initial tetraploidy followed by chromosome loss. Such a mechanism has previously been proposed for a plant tissue culture system (Bayliss andGould 1974) and commonly occurs in animal systems, particularly in animal tumours (Terzi andHawkins 1975). An alternative mechanism, which does not necessarily involve tetraploidy, is also proposed.
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Ashmore, S.E., Gould, A.R. Karyotype evolution in a tumour derived plant tissue culture analysed by Giemsa C-banding. Protoplasma 106, 297–308 (1981). https://doi.org/10.1007/BF01275560
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DOI: https://doi.org/10.1007/BF01275560