Skip to main content

Direct somatic embryogenesis and plantlet regeneration from seedling leaves of winged bean,Psophocarpus tetragonolobus (L.) DC

Plant Cell Reports volume 16, pages 628–631 (1997)Cite this article

Abstract

Excised seedling leaf segments of winged bean [Psophocarpus tetragonolobus (L.) DC.] underwent direct somatic embryogenesis under appropriate incubation conditions. Initiation and development of the somatic embryos occurred using a two-step culture method. The culture procedure involved incubation for 28 days on MS basal medium supplemented with 0.1–0.5 mg/l NAA and 1.0–2.0 mg/l BA (induction medium) before transfer to MS medium supplemented with 0.1 mg/l IAA and 2.0 mg/l BA (embryo development medium). The initial exposure to low levels of NAA coincident with high levels of BA in the induction medium was essential for embryogenic induction. Maximum embryogenesis (43.3%) was obtained with 0.2 mg/l NAA and 2.0 mg/l BA, and at least 14 days on induction medium were required prior to transfer to the embryo development medium. The conversion frequency of cotyledonary embryos was 53.3% upon culture on MS medium containing 0.1 mg/l ABA for 7 days followed by transfer to MS medium supplemented with 0.1 mg/l IBA and 0.2 mg/l BA. Following conversion, the regenerated plantlets were transferred to soil and showed normal morphological characteristics.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Abbreviations

MS :

Murashige and Skoog (1962) medium

2,4-D :

2,4-dichlorophenoxyacetic acid

NAA :

1-naphthaleneacetic acid

IAA :

indole-3-acetic acid

IBA :

indole-3-butyric acid

BA :

6-benzylaminopurine

ABA :

abscisic acid

References

  • Ahmed R, Dutta Gupta S, De DN (1996) Somatic embryogenesis and plant regeneration from leaf derived callus of winged bean [Psophocarpus tetragonolobus (L.) Dc.] Plant Cell Rep 15:531–535

    Google Scholar 

  • Baker CM, Wetzstein HY (1992) Somatic embryogenesis and plant regeneration from leaflets of PeanutArachis hypogaea. Plant Cell Rep 11:71–75

    Google Scholar 

  • Baker CM, Wetzstein HY (1994) Influence of auxin type and concentrations on peanut somatic embryogenesis. Plant Cell Tiss Org Cult 36:361–368

    Google Scholar 

  • Barna KS, Wakhlu AK (1993) Somatic embryogenesis and plant regeneration from callus cultures of chickpea (Cicer arietinum L.). Plant Cell Rep 12:521–524

    Google Scholar 

  • Barna KS, Wakhlu AK (1995) Direct somatic embryogenesis and plantlet regeneration from immature leaflets in chickpea. In Vitro Cell Dev Biol 31P:137–139

    Google Scholar 

  • Buchheim JA, Colburn SM, Ranch SP (1989) Maturation of soybean somatic embryos and the transition to plantlet growth. Plant Physiol 89:768–775

    Google Scholar 

  • Chengalrayan K, Sathaye SS, Hazra S (1994) Somatic embryogenesis from mature embryo-derived leaflets of peanut (Arachis hypogea L.). Plant Cell Rep 13:578

    Google Scholar 

  • Christianson ML, Warnick DA (1985) Temporal requirement for phytohormone balance in the control of organogenesis. In Vitro Dev Biol 112:494–497

    Google Scholar 

  • Finstad K, Brown DCB, Joy K (1993) Characterization of competence during induction of somatic embryogenesis in alfalfa tissue culture. Plant Cell Tis Org Cult 34:125–132

    Google Scholar 

  • Gregory HM, Haq N, Evans PK (1980) Regeneration of plantlets from leaf callus of the winged beanPsophocarpus tetragonolobus (L) DC. Plant Sci Lett 18:395–400

    Google Scholar 

  • Hazara S, Sathaye SS, Mascarenhas AF (1989) Somatic embryogenesis in peanut (Arachis hypogaea). Biotechnology 7:949–951

    Google Scholar 

  • Lazzeri PA, Hildebrand DF, Collins GB (1987) Soybean somatic embryogenesis. Effects of hormones and culture manipulations. Plant Cell Tiss Org Cult 10:197–208

    Google Scholar 

  • Maheswaren G, Williams EG (1984) Direct somatic embryoid formation on immature embryos ofTrifolium repens, T, pratense, andMedicago sativa, and rapid clonal propagation ofT. repens. Ann Bot 54:201–211

    Google Scholar 

  • Malik KA, Saxena PK (1992) Somatic embryogenesis and shoot regeneration from intact seedlings ofPhaseolus acutifolius. P. aures (L) Wilczek,P. coccineus L., andP. wrightii L. Plant Cell Rep 11:163–168

    Google Scholar 

  • McKently A (1991) Direct somatic embryogenesis from axes of mature peanut embryos. In Vitro Cell Dev Biol 27P:197–200

    Google Scholar 

  • Mehta U, Mohan Ram HY (1981) Tissue culture and whole plant regeneration in the winged bean (Psophocarpus tetragonolobus L.). Ann Bot 47:163–166

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Google Scholar 

  • NAS Report (1979) The winged bean. A high protein crop for the tropics. National Academy of Sciences, Washington, D.C.

  • Ozias-Akins P (1989) Plant regeneration from immature embryos of peanut. Plant Cell Rep 8:217–218

    Google Scholar 

  • Parrott WA, Bailey MA, Durham RE, Mathews HV (1992) Tissue culture and regeneration in legumes. In: Moss JP (eds) Biotechnology and crop improvement in Asia. ICRISAT, Patancheru, India, pp 115–148

    Google Scholar 

  • Sellers RM, Southward GM, Phillips GC (1990) Adventitious somatic embryogenesis, from cultured immature zygotic embryos of peanut using soybean as a model system. Crop Sci 30:408–414

    Google Scholar 

  • Suhasini K, Sagare AP, Krishnamurthy KV (1994) Direct somatic embryogenesis from mature embryo axes in chickpea (Cicer arietinum L.) Plant Sci 102:189–194

    Google Scholar 

  • Tran Thanh Van K, Lie-Schricke H. Marcotte JL, Trinh TH (1986) Winged bean [Psophocarpus tetragonolobus (L.) DC.]. In: Bajaj YPS (eds) Biotechnology in agriculture and forestry, vol2: crop 1. Springer, Berlin Heidelberg New York, pp 556–567

    Google Scholar 

  • Venketeswaran S, Dias MADL, Weyers UV (1992) Organogenesis and somatic embryogenesis from callus of winged bean [Psophocarpus tetragonolobus (L.) DC.] Acta Hortic 280:202–206

    Google Scholar 

  • Wetzstein HY, Baker CM (1993) The influence of somatic embryo morphology and histology on germination and conversion in peanut. Plant Sci 92:81–89

    Google Scholar 

  • Wilson VM, Haq N, Evans PK (1985) Protoplast isolation culture and plant regeneration in the winged bean.Psophocarpus tetragonolobus (L.) DC. Plant Sci 41:61–68

    Google Scholar 

Download references

Author information

Affiliations

  1. Department of Agricultural and Food Engineering, Indian Institute of Technology, 721302, Kharagpur, India

    S. Dutta Gupta, R. Ahmed & D. N. De

Authors
  1. S. Dutta Gupta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Ahmed
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. N. De
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by G. Phillips

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Dutta Gupta, S., Ahmed, R. & De, D.N. Direct somatic embryogenesis and plantlet regeneration from seedling leaves of winged bean,Psophocarpus tetragonolobus (L.) DC. Plant Cell Reports 16, 628–631 (1997). https://doi.org/10.1007/BF01275504

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01275504

Key words

  • Psophocarpus tetragonolobus
  • Somatic embryo induction
  • Embryo morphology Plantlets