Abstract
The effect of parameters involved in the transformation efficiency of peanut (Arachis hypogaea L.) seed tissues by direct gene transfer using a helium inflow particle bombardment device was evaluated. Transient gene expression was affected by both particle and DNA amounts, and was positively correlated with gene copy number, as determined byβ-glucuronidase (GUS) activity assays. No influence of plasmid size on GUS gene expression was observed. Transcriptional control of GUS by either the CaMV 35S or the 2S promoter from Brazil nut 2S albumin gene varied with the developmental stage of the seed and was approximately tenfold greater under the influence of the 35S promoter than under the 2S promoter. The gene products of both the Brazil nut methionine-rich 2S albumin and GUS genes under the transcriptional control of the 35S promoter were detected by ELISA assays.
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Ahmed EH, Young CT (1982) Composition, nutrition, and flavor of peanuts. In: Pattee HE, Young CT (eds) Peanut science and technology. American Peanut Research and Education Society, Yoakum, Tex., pp 655–688
Altenbach SB, Simpson RB (1990) Manipulation of methionine-rich protein genes in plant seeds. Tibtech 8:156–160
Altenbach SB, Pearson KM, Meeker G, Staraci LC, Sun SSM (1989) Enhancement of the methionine content of seed proteins by the expression of a chimeric gene encoding a methionine-rich protein in transgenic plants. Plant Mol Biol 13:513–522
Aragão FJL, Grossi de Sá MF, Almeida ER, Gander ES, Rech EL (1992) Particle bombardment-mediated transient expression of a Brazil nut methionine-rich albumin in bean (Phaseolus vulgaris L.). Plant Mol Biol 20:357–359
Aragão FJL, Grossi de Sá MF, Davey MR, Brasileiro ACM, Faria JC, Rech EL (1993) Factors influencing transient gene expression in bean (Phaseolus vulgaris L.) using an electrical particle acceleration device. Plant Cell Rep 12:483–490
Aragão FJL, Barros LMG, Brasileiro ACM, Ribeiro SG, Smith FD, Sanford JS, Faria JC, Rech EL (1996) Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) cotransformed via particle bombardment. Theor Appl Genet 93:142–150
Boote KJ (1982) Growth stages of peanut (Arachis hypogaea). Peanut Sci 9:35–40
Christou P (1993) Philosophy and practice of variety-independent gene transfer into recalcitrant crops. In Vitro Cell Dev Biol 29P:119–124
D'Halluim K, Bonne E, Bossut M, De Beuckeleer M, Leemans J (1992) Transgenic maize plants by tissue electroporation. Plant Cell 4:1495–1505
Finer JJ, Vain P, Jones MW, McMullen MD (1992) Development of the particle inflow gun for DNA delivery to plant cells. Plant Cell Rep 11:323–328
Gander ES, Homstroem K-O, De Paiva GR, De Castro LAB, Carneiro M, Grossi de Sá MF (1991) Isolation, characterization and expression of a gene coding for a 2S albumin gene fromBertholetia excelsa (Brazil nut). Plant Mol Biol 16:437–448
Grossi de Sá MF, Martins de Sá C, Harper F, Coux O, Akhayat O, Pal JK, Florentin Y, Scherrer K (1988) Cytological localization of prosomes as a function of differentiation. J Cell Sci 89:151–165
Grossi de Sá MF, Weinberg DE, Rech EL, Barros LMG, Aragão FJL, Holmstroem K-O, Gander ES (1994) Functional studies on a seed-specific promoter from a Brazil nut 2S gene. Plant Sci 103:189–198
Haughn GW, Smith J, Mazur B, Somerville C (1988) Transformation with a mutantArabidopsis acetolactate synthase gene renders tobacco resistant to sulphonylurea herbicides. Mol Gen Genet 211:266–271
Hunold R, Bronner R, Hahne G (1994) Early events in microprojectile bombardment: cell viability and particle location. Plant J 5:593–604
Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5:387–405
Klein TM, Gradziel T, Fromm ME, Sanford JC (1988) Factors influencing gene delivery into Zea mays cells by high-velocity microprojectiles. Bio/Technol 6:559–563
McCabe DE, Swain WF, Martineli BJ, Christou P (1988) Stable transformation of soybean (Glycine max) by particle acceleration. Bio/Technol 6:923–926
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Ozias-Akins P, Schnall JA, Anderson WF, Singsit C, Clemente TE, Adang MJ, Weissinger AK (1993) Regeneration of transgenic peanut plants from stably transformed embryogenic callus. Plant Sci 93:1–2
Russel JA, Roy MK, Sanford JC (1992) Major improvements in biolistic transformation of suspension-cultured tobacco cells. In Vitro Cell Dev Biol 28P:97–105
Russell DR, Wallace KM, Bathe JH, Martinell BJ, McCabe DE (1993) Stable transformation ofPhaseolus vulgaris via eletricdischarge mediated particle acceleration. Plant Cell Rep 12:165–169
Saalbach I, Waddell D, Pickard T, Schieder O, Müntz K (1995) Stable expression of the sulphur-rich 2S albumin gene in transgenicVicia narbonensis increases the methionine content of seeds. J Plant Physiol 145:674–681
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning - a laboratory manual, 2nd edn, vol. 1. Cold Spring Harbour Laboratory Press, New York
Sanford JC, Smith FD, Russel JA (1993) Optimizing the biolistic process for different biological applications. Methods Enzymol 217:483–510
Schledzewski K, Mendel RR (1994) Quantitative transient gene expression: comparison of the promoters for maize polyubiquitin 1, rice actin 1, maize-derivedEmu and CaMV35S in cells of barley, maize and tobacco. Transgen Res 3:249–255
Yamashita T, Iida A, Morikawa H (1991) Evidence that more than 90% ofβ-glucuronidase-expressing cells after particle bombardment directly receive the foreign gene in their nucleus. Plant Physiol 97:829–831
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Communicated by J. F. Finer
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Lacorte, C., Aragão, F.J.L., Almeida, E.R. et al. Transient expression of GUS and the 2S albumin gene from Brazil nut in peanut (Arachis hypogaea L.) seed explants using particle bombardment. Plant Cell Reports 16, 619–623 (1997). https://doi.org/10.1007/BF01275502
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DOI: https://doi.org/10.1007/BF01275502