Abstract
TwoTaxus (T. chinensis andT. baccata) cell suspension cultures were used as a model system to demonstrate the similarities of biomass accumulation and secondary metabolite (taxane) production obtained from cultures in six-well polystyrene plates and glass shake flasks (25 ml and 125 ml). Interference from binding of taxanes in cell-free culture broth to the polystyrene plates was minimal with 85% of the paclitaxel (Taxol®) and 100% of baccatin and 10-deacetyl-7-xylosyl-taxol remaining in the medium after 24 h beyond which no further binding was observed. A simple thin layer chromatography (TLC) procedure with a chloroform: acentonitrile (4:1) solvent system on silica gel was developed to simultaneously test up to 17 cultures for taxane production. The combination of six-well plate technology for experimentation and TLC for rapid taxane analysis can greatly accelerate the establishment of conditions for an optimalTaxus plant-cell culture process for taxane production.
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Abbreviations
- TLC :
-
Thin layer chromatography
- 2,4D :
-
2,4-dichlorophenoxyacetic acid
- HPLC :
-
high pressure liquid chromotography
- UV :
-
ultraviolet
- Rf :
-
retention factor
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Communicated by F. Constabel
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Srinivasan, V., Roberts, S.C. & Shuler, M.L. Combined use of six-well polystyrene plates and thin layer chromatography for rapid development of optimal plant cell culture processes: application to taxane production byTaxus sp.. Plant Cell Reports 16, 600–604 (1997). https://doi.org/10.1007/BF01275499
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DOI: https://doi.org/10.1007/BF01275499