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Cryopreservation of invitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott) by vitrification. 1. Investigation of basic conditions of the vitrification procedure

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Abstract

Invitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott.) were successfully cryopreserved by vitrification. Excised shoot tips precultured on solidified MS supplemented with 0.3M sucrose and maintained under a 16 h phtoperiod at 25°C for 16 h were loaded with a mixture of 2M glycerol plus 0.4M sucrose for 20 min at 25°C. The shoot tips were then sufficiently dehydrated with a highly concentrated vitrification solution (PVS2) for 20 min at 25°C prior to immersion into liquid nitrogen. Successfully vitrified and warmed shoot tips resumed growth within 7 days and developed shoots directly without intermediate callus formation. The average rate of shoot recovery amounted to around 80%, and the vitrification protocol appeared to be very promising for the cryopreservation of taro germplasm.

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Abbreviations

DMSO :

Dimethylsulfoxide

EG :

ethylene glycol

LN :

liquid nitrogen

MS :

Murashige & Skoog medium (1962)

TDZ :

thidiazuron

References

  • Dereuddre J, Fabre J, Bassaglia C (1988) Resistance to freezing in liquid nitrogen of carnation (Dianthus caryophyllus L. var ‘Eolo’) apical and axillary shoot tips excised from different aged in vitro plantlets. Plant Cell Rep 7:170–173

    Google Scholar 

  • Dereuddre J, Scottez C, Amud Y, Duron M (1990) Resistance of alginate-coated axillary shoot tips of pear tree (Pyrus communis L. cv ‘Beurré Hardy’) in vitro plantlets to dehydration and subsequent freezing in liquid nitrogen: effects of previous cold hardening. C R Acad Sci Paris t 310 Ser III: 317–323

    Google Scholar 

  • Engelmann F (1991)In vitro conservation of tropical plant germplasm — a review. Euphytica 57:227–243

    Google Scholar 

  • Fabre J, Dereuddre J (1990) Encapsulation-dehydration: a new approach to cryopreservation ofSolanum shoot-tips. Cryo-Lett 11:413–426

    Google Scholar 

  • Langis R, Schnabel-Preikstas ED, Steponkus PL (1990) Cryopreservation of carnation shoot tips by vitrification. Cryobiology 27:657–658

    Google Scholar 

  • Matsumoto T, Sakai A, Yamada K (1994) Cryopreservation of in vitro-grown apical meristems of wasabi (Wasabia japonica) by vitrification and subsequent high plant regeneration. Plant Cell Rep 13:442–446

    Google Scholar 

  • Matsumoto T, Sakai A, Takahashi C, Yamada K (1995a) Cryopreservation of in vitro grown apical meristems of lily by vitrification. Plant Cell Tissue Org Cult 41:237–241

    Google Scholar 

  • Matsumoto T, Sakai A, Takahashi C, Yamada K (1995b) Cryopreservation of in vitro-grown apical meristems of wasabi (Wasabia japonica) by encapsulation-vitrification method. Cryo-Lett 16:189–196

    Google Scholar 

  • Niino T, Sakai A, Yakuwa H, Nojiri K (1992) Cryopreservation of in vitro-grown shoot tips of apple and pear by vitirification. Plant Cell Tissue Org Cult 28:261–266

    Google Scholar 

  • Panis B (1995) Cryopreservation of banana (Musa spp.) germplasm. PhD thesis, Thesis No. 272, Katholieke Universiteit, Leuven, Belgium

    Google Scholar 

  • Purseglove JW (1972) Araceae. In: Purseglove JW (ed) Tropical monocotyledons. The English Language Book Society And Longman, Harlow, pp 58–74

    Google Scholar 

  • Sakai A, Kobayashi S, Oiyama, I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var ‘brasiliensis Tanaka’) by vitrification. Plant Cell Rep 9:30–33

    Google Scholar 

  • Shimonishi K, Karube M, Ishikawa M (1993) Cryopreservation of taro (Colocasia esculenta) embryogenic callus by slow prefreezing (in Japanese). Jpn J Breed 43[Suppl 2]:187

    Google Scholar 

  • Takagi H, Otoo E, Islam OM, Senboku T (1994) In vitro preservation of germplasm in root and tuber crops. I. Preliminary investigation of mid- and long-term preservation of yams (Dioscorea spp.) and taro (Colocasia esculenta (L.) Shott) (in Japanese). Breed Sci 44[Suppl 1]:273

    Google Scholar 

  • Towill LE (1990) Cryopreservation of isolated mint shoot tips by vitrification. Plant Cell Rep 9:178–180

    Google Scholar 

  • Towill LE, Jarret RL (1992) Cryopreservation of sweet potato [Ipomoea batatas (L.) Lam.] shoot tips by vitrification. Plant Cell Rep 11:175–178

    Google Scholar 

  • Yamada T, Sakai A, Matsumura T, Higuchi S (1991) Cryopreservation of apical meristems of white clover (Trifolium repens L.) by vitrification. Plant Sci 78:81–87

    Google Scholar 

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Author notes

  1. N. Tien Thinh

    Present address: Nuclear Research Institute, Dalat, Viet Nam

  2. O. M. Islam

    Present address: Plant Genetic Resources Center, Bangladesh Agriculture Research Institute, Bangladesh

  3. T. Senboku

    Present address: Agriculture, Forestry and Fisheries Research Council Secretariat, MAFF, Tokyo, Japan

Authors and Affiliations

  1. Japan International Research Center for Agricultural Sciences, Okinawa Subtropical Station, 1091-1 Kawarabaru, Maesato, Ishigaki city, 907, Okinawa, Japan

    H. Takagi, N. Tien Thinh, O. M. Islam & T. Senboku

  2. Asabucho 1-5-23, Kitaku, 001, Sapporo, Japan

    A. Sakai

Authors
  1. H. Takagi
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  2. N. Tien Thinh
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  3. O. M. Islam
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  4. T. Senboku
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  5. A. Sakai
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Communicated by A. Kornamine

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Takagi, H., Tien Thinh, N., Islam, O.M. et al. Cryopreservation of invitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott) by vitrification. 1. Investigation of basic conditions of the vitrification procedure. Plant Cell Reports 16, 594–599 (1997). https://doi.org/10.1007/BF01275498

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  • DOI: https://doi.org/10.1007/BF01275498

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