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Partial auxin deprivation affects endogenous cytokinins in an auxin-dependent, cytokinin-independent tobacco cell strain

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Abstract

The dynamics of individual endogenous cytokinins within the growth cycle (subculture interval) of an auxin-dependent and cytokinin-independent cell suspension culture ofNicotiana tabacum L. (strain VBI-0) were determined using high performance liquid chromatography and radioimmunoassay. In cells grown at an optimum auxin concentration the transient maxima of N6-(Δ2-isopentenyl)adenine and N6-(Δ2-isopentenyl)-adenosine correlated with the onset of cell division. Cultivation of the cells in a partially auxin-deprived medium resulted in ca. tenfold increase of all endogenous cytokinins. A very distinct maximum of N6-(Δ2-sopentenyl) adenine appeared at the beginning of subculture. This indicates that a lack of auxin induced an accumulation of cytokinins predominantly in the form of the free bases, which are physiologically more active than the corresponding ribosides.

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Abbreviations

iP:

N6-(Δ2-isopentenyl)adenine

[9R]iP:

N6-(Δ2-isopentenyl)adenosine

t-Z :

trans-zeatin

t-[9R]Z:

trans-zeatin riboside

IAA:

indole-3-acetic acid

NAA:

1-naphthaleneacetic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

f.w.:

fresh weight

SBI:

subculture interval

C:

complete medium

PAD:

partially auxin-deprived medium

RP-HPLC:

reverse phase high performance liquid chromatography

RIA:

radioimmunoassay

PAL:

L-phenylalanine ammonia lyase

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Authors and Affiliations

  1. De Montford University Norman Borlaug Centre for Plant Science, Institute of Experimental Botany ASCR, Ke dvoru 16/15, 166 30, Prague 6, Czech Republic

    Eva Zažímalová

  2. Institute of Experimental Botany ASCR, Ke dvoru 16/15, 166 30, Prague 6, Czech Republic

    Alena Březinová & Josef Holík

  3. Research Institute of Crop Production, Drnovská 507, 16100, Prague 6 - Ruzyně, Czech Republic

    Zdeněk Opatrný

Authors
  1. Eva Zažímalová
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  2. Alena Březinová
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  3. Josef Holík
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  4. Zdeněk Opatrný
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Communicated by I. Potrykus

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Zažímalová, E., Březinová, A., Holík, J. et al. Partial auxin deprivation affects endogenous cytokinins in an auxin-dependent, cytokinin-independent tobacco cell strain. Plant Cell Reports 16, 76–79 (1996). https://doi.org/10.1007/BF01275454

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  • DOI: https://doi.org/10.1007/BF01275454

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