Summary
A suspension of tobacco cells,Nicotiana tabacum L. BY-2, was subjected to a rapid change of medium, resulting in disturbance of growth. A subpopulation of growing cells responded to such a nutritional signal by establishing a transient, localized Ca2+ accumulation, as judged by chlorotetracycline fluorescence. Residing near or at the plasma membrane, this initial Ca2+ signal began to relax after 1 h to a value presumably corresponding to an equilibrium Ca2+ level. This response was susceptible to treatment with brefeldin A, an agent impacting vesicular traffic, as indicated by a further increase in fluorescence. By contrast, undisturbed growing and non-growing cells did not display a Ca2+ response, regardless of the presence of brefeldin A.
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Communicated by I. K. Vasil
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Vitorello, V.A., Haug, A. Growing tobacco cells respond to rapid medium changes with a transient increase in localized Ca2+ activity. Plant Cell Reports 16, 63–66 (1996). https://doi.org/10.1007/BF01275451
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DOI: https://doi.org/10.1007/BF01275451