Summary
The biological effects of irradiation with12C+5 ion beam on plant cells have been analyzed. Protoplasts and cell suspensions ofLavatera thuringiaca, and a somatic hybrid callus (Hibiscus rosa-sinensis +Lavatera thuringiaca), were irradiated with doses from 0.05 to 50 Gy, and the effects on cell growth, cell division, cell viability and embryogenesis rates were analyzed. Irradiation with12C+5 ion beam at relatively very low doses (5.0 Gy) significantly inhibited cell division, yet the survival rate and regeneration capability of the cells through somatic embryogenesis were conserved in more than 70 and 50 %, respectively. These results indicate that cell division is the most sensitive parameter to irradiation, accounting for the inhibition of colony formation and callus growth. The potential use of the12C+5 ion beam in asymmetric protoplast fusion experiments is discussed.
Similar content being viewed by others
References
Aviv D, Galun E (1980) Theor Appl Genet 58:121–127
Bates GW, Hasenkampf CA, Contolini CL, Piastuch WC (1987) Theor Appl Genet 74:718–726
Imamura J, Saul MW, Potrykus J (1987) Theor Appl Genet 74:445–450
Koorneef M, Dellaert LWM, van der Veen JH (1982) Mutation Res 93:109–123
Koorneef M, van Eden J, Hanhart CJ, de Jongh AMM (1983) Genet Res Camb 41:57–68
Menczel L, Galiba G, Nagy F, Maliga P (1982) Genetics 100:487–495
Sacristan MD, Gerdemann-Knorck M, Schieder O (1989) Theor Appl Genet 78:194–200
Shirley BW, Hanley S, Goodman HM (1992) The Plant Cell 4:333–347
Sidorov VA, Menczel L, Nagy F, Maliga P (1981) Planta 152:341–345
Sidorov VA, Zubko MK, Kuchko YY, Komarnitsky IK, Gleba YY (1987) Theor Appl Genet 74:364–368
Sun TP, Goodman HM, Ausubel FM (1992) The Plant Cell 4:119–128
Sung ZR, Okimoto R (1983) Proc Natl Acad Sci USA 80:2661–2665
Thomzik JE, Hain R (1988) Theor Appl Genet 76:165–171
Vazquez-Tello A, Hidaka M, Uozumi T (1995) Plant Cell Tissue Organ Culture 40:169–177
Vazquez-Tello A, Yang LJ, Hidaka M, Uozumi T (1996) Plant Cell Reports 15: 506–511
Walters TW, Mutschler MA, Earle ED (1992) Plant Cell Reports 10:624–628
Author information
Authors and Affiliations
Additional information
Communicated by A. Komamine
Rights and permissions
About this article
Cite this article
Vazquez-Tello, A., Uozumi, T., Hidaka, M. et al. Effect of12C+5 ion beam irradiation on cell viability and plant regeneration in callus, protoplasts and cell suspensions ofLavateva thuringiaca . Plant Cell Reports 16, 46–49 (1996). https://doi.org/10.1007/BF01275447
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01275447