Skip to main content
SpringerLink
Log in

Genetic transformation ofRhododendron byAgrobacterium tumefaciens

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Summary

TransgenicRhododendron plants were obtained byAgrobacterium tumefaciens-mediated gene transfer.A. tumefaciens harboring a binary vector that contained the chimeric neomycin phosphotransferase II (NPTII) and (3-glucuronidase (GUS) genes was co-cultivated with stem and leaf segments fromRhododendron tissues culturedin vitro. Adventitious buds were fonned and shoots were regenerated on kanamycin selection medium 3-4 months after inoculation. Integration of the NPTII and the GUS genes was confirmed by polymerase chain reaction (PCR) and by Southern hybridization analyses. Histochemical GUS assay showed that the inserted gene was expressed in all tissues with the cauliflower mosaic virus (CaMV) 35S promoter. This transformation procedure has the potential to expand the range of genetic variation inRhododendron.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anderson WC (1984) J Amer Soc Hort Sci 109:343–347

    Google Scholar 

  • Beck EL, Ludwig G, Averswald EA, Reiss B, Schaller H (1982) Gene 19:327–336

    PubMed  Google Scholar 

  • Bevan M, Barnes WM, Chilton MD (1983) Nucleic Acids Research 11(2)

  • Dai C, Lambeth CV, Tavern R, Mertz D (1987) HortScience 22: 491–493

    Google Scholar 

  • Economou AS, Read PE, Spanoudaki MJ (1987) Acta Hort 226: 209–216

    Google Scholar 

  • Fordhman I, Stimart DP, Zimmerman RH (1982) HortScience 17: 738–739

    Google Scholar 

  • Harbage JF, Stimart DP (1987) HortScience 22: 1324–1325

    Google Scholar 

  • Hidaka T, Omura M, Ugaki M, Tomiyama M, Kato A, Ohshima M, Motoyoshi F (1990) Japan J Breed 40: 199–207

    Google Scholar 

  • Hoekema A, Hirsch PR, Hooykass PJJ, SchiIperoort RA (1983) Nature 303:179–180

    Google Scholar 

  • Honda H, Hirai A (1990) Japan J Breed 40:339–348

    Google Scholar 

  • Iapichino G, Chen THH, Fuchigami LH (1991) Plant Cell Tiss Org Cult 27: 37–43

    Google Scholar 

  • James DJ, Passey AJ, Barbara DJ, Bevan M (1989) Plant Cell Reports 7:658–661

    Google Scholar 

  • Jefferson RA, Burgess SM, Hirsh D (1986) Proc Natl Acad Sci USA 83: 8447–8451

    PubMed  Google Scholar 

  • Jefferson RA, Kavanagh TA, Bevan MW (1987) EMBO J 6(13): 3901–3907

    PubMed  Google Scholar 

  • Kosugi S, Ohashi Y, Nakajima K, Arai Y (1990) Plant Sci 70:133–140

    Google Scholar 

  • Kurioka Y, Suzuki Y, Kamada H, Harada H (1992) Plant Cell Reports 12:1–6

    Google Scholar 

  • McGranahan GH, Leslie CA, Uratsu SL, Martin LA, Dandekar AM (1988) Bio/Technology 6: 800–804

    Google Scholar 

  • Meyer MM (1982) HortScience 17: 891–892

    Google Scholar 

  • Murakami T, Ohashi Y (1992) Plant Cell Tech 4: 281–286

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15: 473–497

    Google Scholar 

  • Takagi H, Tanaka Y, Tarumoto I, Murata N (1993) Japan J Breed 43 (Suppl. 1): 192

    Google Scholar 

  • Vervliet G, Holsters M, Teuchy H, Van Montagu M, Schell J (1975) J Gen Virol 26:33–48

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Kagoshima Biotechnology Institute, 893-16, Kushira, Kagoshima, Japan

    Kei-ichiro Ueno

  2. Takii Agricultural Experimental Station, 520-32, Kousei, Shiga, Japan

    Yutaka Fukunaga

  3. Faculty of Agriculture, Kagoshima University, Kagoshima 890, Japan

    Ken-ichi Arisumi

Authors
  1. Kei-ichiro Ueno
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yutaka Fukunaga
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ken-ichi Arisumi
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by J. J. Finer

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ueno, Ki., Fukunaga, Y. & Arisumi, Ki. Genetic transformation ofRhododendron byAgrobacterium tumefaciens . Plant Cell Reports 16, 38–41 (1996). https://doi.org/10.1007/BF01275445

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01275445

Keywords

Search

Navigation