Abstract
Gynogenetic plants of pot gerbera (Gerbera jamesonii) were successfully produced from cultures of unpollinated ovulesin vitro. Genotypic variations in the number of ovules that formed callus were found among the lines tested. One particularly responsive genotype was found among 17 genotypes tested where the frequency of callus-forming ovules was 17.5%. Four genotypes formed no callus at all. The frequency of shoot formation from the callus varied from 0–19.6% in nine genotypes. Ploidy was determined by flow cytometry, and 37 (80.4%) regenerants were haploid, seven (15.2%) were diploid, and two (4.3%) were mixoploid with both haploid and diploid cells. The doubling of chromosomes was achieved by treatment with 0.05% colchicine for 2–6 din vitro, and 24.2–34.1% of treated haploid plants were found to have been diploidized.
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Abbreviations
- BA:
-
6-benzylaminopurine
- NAA:
-
1-naphthaleneacetic acid
- IAA:
-
indole-3-acetic acid
- DAPI:
-
4′ ,6-diamidino-2-phenylindole dihydrochloride
- MS:
-
Murashige and Skoog (1962) basal medium
References
Alemanno L, Guiderdoni E (1994) Plant Cell Reports 13: 432–436
Ahmim M, Vieth J (1986) Can J Bot 64: 2355–2357
Behnke M (1991) In: Ball V (ed) The Ball RedBook, 15th ed, Greenhouse growing. Geo J Ball Publishing, West Chicago, IL, pp 555–559
Cagnet-Sitbon M (1981) Agronomic 1: 807–812
Cappadocia M, Chretien L, Laublin G (1988) Can J Bot 66: 1107–1110
Cappadocia M, Vieth J (1990) In: Bajaj YPS (ed), Biotechnology in Agriculture and Forestry, vol 12, Haploids in crop improvement I. Springer-Verlag, Berlin Heidelberg
De Laat AMM, Gohde W, Vogelzang JDC (1987) Plant Breeding 99: 303–307
Fahleson J, Dixelius J, Sundberg E, Glimelius K (1988) Plant Cell Reports 7: 74–77
Heller R (1953) Ann Sci Nat Biol Veg 14: 1–123
Honkanen J, Aapola A, DeWit JC, Esendam HF, Seppanen P, Tormala T, Stravers LIM (1991) Acta Hortic 300: 341–346
Meynet J, Sibi M (1984) Z Pflanzenzuchtg 93: 78–85
Murashige T, Skoog F (1962) Physiol Plant 15:473–497
Nishibayashi S (1990) Plant Tissue Cult Lett (Tokyo) 7: 127–129
Rogers MN, Tjia BO (1990) Gerbera production for cut flowers and pot plants: Growers handbook series, vol 4. Timber Press, Portland, OR
Tosca A, Lombardi M, Marinoni L, Conti L, Frangi P (1990) Acta Hort 280: 337–340
Tosca A, Pandolfi R, Citterio S, Fasoli A, Sogorbati S (1995) Plant Cell Reports 14: 455–458.
Wan Y, Duncan DR, Rayburn AL, Petolino JF, Widholm JM (1991) Theor Appl Genet 81: 205–211
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Communicated by G. Phillips
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Miyoshi, K., Asakura, N. Callus induction, regeneration of haploid plants and chromosome doubling in ovule cultures of pot gerbera (Gerbera jamesonii). Plant Cell Reports 16, 1–5 (1996). https://doi.org/10.1007/BF01275438
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DOI: https://doi.org/10.1007/BF01275438