Summary
Labelling and inhibitor studies on LP, SP, and lateral root apical meristems have demonstrated the presence of the enzymes TdR kinase and TMP synthetase in active form in all three proliferating tissues. A permeability barrier in the epidermis and cortex of the primary root and also the fluid filled cavity between LP and the cortex prevent much exogenously supplied deoxynucleoside from reaching these cells. Values have been obtained for the duration of G2 in both apical meristems of lateral roots and SP, and, by using data previously reported elsewhere, the durations of all phases of the mitotic cycle in SP have been calculated.
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Ashford, A. E., andM. E. McCully, 1970: Localization of naphthol AS-B1 phosphate activity in lateral and main root meristems of pea and corn. Protoplasma70, 441–456.
Bonnett, H. T., 1969: Cortical cell death during lateral root formation. J. Cell Biol.40, 144–159.
Cohen, S. S., J. C. Flaks, H. D. Barner, M. R. Loeb, andJ. Lichtenstein, 1958: The mode of action of 5-fluorouracil and its derivatives. Proc. natl. Acad. Sci. (U.S.)44, 1004–1012.
Davidson, D., 1961: Mechanisms of reorganisation and cell repopulation in meristems in roots ofVicia faba following irradiation and colchicine. Chromosoma12, 484–504.
—, 1965: A differential response to colchicine of meristems of roots ofVicia faba. Ann. Bot.29, 253–264.
— andR. D. Macleod, 1966: Changes in mitotic indices in roots ofVicia faba. I. Antagonistic effects of colchicine and IAA. Chromosoma18, 421–437.
— —, 1968: Response of meristems to indoleacetic acid and colchicine: Differences between primordia and root meristems. Bot. Gaz.129, 166–171.
Friedberg, S., 1969: A study of root morphogenesis using mixoploid cell populations. Ph. D. thesis, Case Western Reserve University, Cleveland, Ohio.
Hartmann, K.-U., andC. Heidelberger, 1961: Studies on fluorinated pyrimidines. XIII. Inhibition of thymidylate synthetase. J. Biol. Chem.236, 3006–3013.
Hoffmann, G. R., 1970: Studies on the reversal of 5-fluorodeoxyuridine-induced mitotic inhibition by thymidine and thymidine analogues. Canad. J. Genet. Cytol.12, 230–240.
Hummon, M. R., 1962: The effects of tritiated thymidine incorporation on secondary root production byPisum sativum. Amer. J. Bot.49, 1038–1046.
Macleod, R. D., 1968: Changes in the mitotic cycle in lateral root meristems ofVicia faba following kinetin treatment. Chromosoma24, 177–187.
—, 1971: Thymidine kinase activity and its relationship to DNA synthesis in the primary root ofVicia faba. Ann. Bot.35, 237–248.
— andD. Davidson, 1968: Delayed incorporation of H3-thymidine by primordial cells. Chromosoma24, 1–9.
— —, 1970: Incorporation of3H-deoxynucleosides: changes in labelling indices during root development. Canad. J. Bot.48, 1659–1663.
- C.Preston, 1971: unpublished data.
Mahler, H. R., andE. H. Cordes, 1969: Biological Chemistry, 3rd Edition, New York: Harper.
Okazaki, R., andA. Kornberg, 1964: Deoxythymidine kinase ofEscherichia coli. I. Purification and some properties of the enzyme. J. Biol. Chem.239, 269–274.
Quastler, H., andF. G. Sherman, 1959: Cell population kinetics in the intestinal epithelium of the mouse. Exp. Cell Res.17, 420–438.
Smillie, R. M., andG. Krotkov, 1960: The estimation of nucleic acids in some algae and higher plants. Canad. J. Bot.38, 31–49.
Socher, S. H., andD. Davidson, 1970: Heterogeneity in G2 duration during lateral root development. Chromosoma31, 478–484.
Webster, P. L., andD. Davidson, 1968: Evidence from thymidine-3H-labeled meristems ofVicia faba of two cell populations. J. Cell Biol.39, 332–338.
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Macleod, R.D. Thymidine kinase and thymidylate synthetase in meristems of roots ofVicia faba . Protoplasma 73, 337–348 (1971). https://doi.org/10.1007/BF01273938
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DOI: https://doi.org/10.1007/BF01273938