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Counting of gas vacuoles by electron microscopy in lysates and purified fractions ofmicrocystis aeruginosa

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Summary

A microdroplet spray method is described to determine quantitatively the number of gas vacuoles in purified fractions and in total lysates of the blue-green alga,Microcystis aeruginosa.

It is found that 6.45×1012 gas vacuoles of 370 nm length make up one mg of protein. This supports the idea that the entire organelle is made up by a single layer of protein only. The gas vacuole number per cell varies with the stage of growth between 3,700 and 11,000.

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Author notes

  1. Michael Jost

    Present address: MSU/AEC Plant Research Laboratory and Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan, USA

  2. Daniel D. Jones

    Present address: College of General Studies, University of Alabama, 35233, Birmingham, AL, USA

Authors and Affiliations

  1. MSU/AEC Plant Research Laboratory and Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan, USA

    Daniel D. Jones & P. J. Weathers

Authors
  1. Michael Jost
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  2. Daniel D. Jones
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  3. P. J. Weathers
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Jost, M., Jones, D.D. & Weathers, P.J. Counting of gas vacuoles by electron microscopy in lysates and purified fractions ofmicrocystis aeruginosa . Protoplasma 73, 329–335 (1971). https://doi.org/10.1007/BF01273937

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  • DOI: https://doi.org/10.1007/BF01273937

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