Summary
Viable megaspore protoplasts ofTorenia fournieri were isolated with enzymatic digestion and mild osmotic shock. With this method, living haploid protoplasts were released from the ovules, while very few sporophytic protoplasts were produced.
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References
Ao GM, Zhao SX, Li GH (1982) In vitro induction of haploid plantlets from unpollinated ovaries of corn (Zea mays L.). Acta Genet Sin 9: 281–283
Asselin de Beauville M (1980) Obtention d'haploides in vitro a patir d'ovaries non fecondes de riz,Oryza saliva L. C R Acad Sci 296D: 489–492
Cai DT, Zhou C (1984) In vitro induction of haploid embryoids and plantlets from unpollinated young florets and ovules ofHelianthus annuus L. Kexue Tongbao 29: 680–682
Campenot MK, Zhang G, Cutler AJ, Cass DD (1992) Zea mays embryo sacs in culture. I. Plant regeneration from 1 day after pollination embryos. Am J Bot 79: 1368–1373
Gu ZP, Cheng KC (1983) In vitro induction of haploid plantlets from unpollinated young ovaries of lily and its embryological observations. Acta Bot Sin 25: 24–28
Heslop-Harrison J, Heslop-Harrison Y, Shivanna KR (1984) The evaluation of pollen quality and a further appraisal of the fluorochromatic (FCR) test procedure. Theor Appl Genet 67: 367–375
Hu S, Li L, Zhu C (1985) Isolation of viable embryo sacs and their protoplasts ofNicotiana tabacum. Acta Bot Sin 27: 337–344
Kovács M, Barnabás B, Kranz E (1994) The isolation of viable egg cells of wheat (Triticum aestivum L.). Sex Plant Reprod 7: 311–312
Kranz E, Lörz H (1993) In vitro fertilisation with isolated, single gametes results in zygotic embryogenesis and fertile maize plants. Plant Cell 5: 739–746
Mól R (1986) Isolation of protoplasts from female gametophytes ofTorenia fournieri. Plant Cell Rep 3: 202–206
Mól R, Matthys-Rochon E, Dumas C (1993) In-vitro culture of fertilised embryo sacs of maize: zygotes and two-celled proembryos can develop into plants. Planta 189: 213–217
San Noeum LH (1976) Haploides d'Hordeum vulgare L. par culture in vitro non fecondes. Ann Amelior Plant 26: 751–754
Tiwari SC (1982) Callose in the walls of mature embryo sac ofTorenia fournieri. Protoplasma 110: 1–4
Zhou C, Yang HY (1980) In vitro induction of haploid plantlets from unpollinated young ovaries ofOrzyza sativa L. Acta Genet Sin 7: 287–288
Zhou C, Yang HY (1984) Enzymatic isolation of embryo sacs from fixed and fresh ovules inAntirrhinum majus L. Acta Biol Exp Sin 17: 141–147
— — (1985) Observations on enzymatically isolated, living and fixed embryo sacs in several angiosperm species. Planta 165: 225–231
Zhu ZC, Wu HS (1979) In vitro induction of haploid plantlets from ovaries ofTriticum aestivum andNicotiana tabacum. Acta Genet Sin 23: 499–501
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Kristóf, Z., Imre, K. Isolation of living megaspores ofTorenia fournieri . Protoplasma 192, 245–248 (1996). https://doi.org/10.1007/BF01273896
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DOI: https://doi.org/10.1007/BF01273896