Summary
Transcription and RNA processing are main functions of the nucleus. These processes are found localized in specific nuclear domains. We have investigated the presence of nuclear transcription domains in plants by incorporating 5-bromouridine 5′-triphosphate in nascent RNA in isolated nuclei ofBrassica napus. Bromo-uridine labelled RNA was visualized by a FITC-labelled biotin-avidin system in combination with confocal laser scanning microscopy. Labelled domains were found throughout the nucleus, in some cases including the nucleolus. This shows that the distribution of transcription sites in plant nuclei is similar to that of mammalian nuclei and that the same labelling procedures can be used.
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Straatman, K.R., Trompetter, C.M., Schul, W. et al. Fluorescent labelling of nascent RNA reveals nuclear transcription domains throughout plant cell nuclei. Protoplasma 192, 145–149 (1996). https://doi.org/10.1007/BF01273886
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DOI: https://doi.org/10.1007/BF01273886