Summary
Rapid activation of blood platelets is required for effective haemostasis, with shape change, aggregation, secretion of granule contents and cell adhesion occurring in seconds or even milliseconds. Signal-transduction events, evidenced by changes in protein phosphorylation and calcium levels, also take place in this time domain. We have now shown that platelet adhesion to collagen via the α2β1 integrin under arterial shear forces initiated the rapid dephosphorylation of a 67 kDa protein “band” which contained the 70 kDa constitutive heat-shock protein, hsc70. Immunoprecipitation with hsc70 antibodies revealed a large phosphoprotein complex in resting platelets and adhesion caused dissociation of the complex along with dephosphorylation of hsc70. The complex also contained the hsp90 heat-shock protein, protein phosphatase 1C, α, δ and M subunits, and some 7–8 unidentified phosphoproteins. The data suggest that heat-shock proteins and protein phosphatases are actively involved in integrin-mediated platelet adhesion.
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Gear, A.R.L., Simon, C.G. & Polanowska-Grabowska, R. Platelet adhesion to collagen activates a phosphoprotein complex of heat-shock proteins and protein phosphatase 1. J. Neural Transmission 104, 1037–1047 (1997). https://doi.org/10.1007/BF01273317
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DOI: https://doi.org/10.1007/BF01273317