Summary
The microtubule distribution during the transition from interphase to the mitotic phase was examined at ultrastructural level in large highly vacuolated cells ofNautilocalyx lynchii and in small non-vacuolated cells ofPisum sativum. Both cell types contain, besides preprophase bands and perinuclear microtubules, also microtubules radiating from the nucleus into the transvacuolar cytoplasmic strands and cytoplasm respectively.
This microtubule array appears to be nucleated by the cell's nuclear envelope (NE) or NE-surrounding cytoplasm.
It is hypothesized that the microtubules radiating from the nucleusinitially play a role in the mobilization of the nucleus whilelater on a stabilized part of this array anchors the nucleus in the plane of cell division, and thus forms a cytoskeletal link between nucleus and division site.
Our results are discussed in the light of previous work on cytoplasmic behaviour during interphase-mitosis transition in highly vacuolated plant cells.
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References
Burgess, J., Northcote, D. H., 1967: A function of the preprophase band of microtubules inPhleum pratense. Planta75, 319–326.
—, 1970: Interactions between microtubules and the nuclear envelope during mitosis in a fern. Protoplasma71, 77–89.
Galatis, B., Mitrakos, K., 1979: On the differential divisions and preprophase microtubule bands involved in the development of stomata ofVigna sinensis L. J. Cell Sci.37, 11–37.
—,Apostolakos, P., Katsaros, Chr., Loukari, H., 1982: Preprophase microtubule band and local wall thickening in guard cell mother cells of someLeguminosae. Ann. Bot.50, 779–791.
Goosen-de Roo, L., Bakhuizen, R., Spronsen, P. C. van, Libbenga, K. R., 1984: The presence of extended phragmosomes containing cytoskeletal elements in fusiform cambial cells ofFraxinus excelsior L. Protoplasma122, 145–152.
Gunning, B. E. S., Hardham, A. R., Hughes, J. E., 1978: Preprophase bands of microtubules in all categories of formative and proliferative cell division inAzolla roots. Planta143, 145–160.
—, 1982: The cytokinetic apparatus: its development and spatial regulation. In: The cytoskeleton in plant growth and development (Lloyd, C. W., ed.), pp. 229–292. London: Academic Press.
Ledbetter, M. C., Porter, K. R., 1963: A “microtubule” in plant cell fine structure. J. Cell Biol.19, 239–250.
Lloyd, C. W., Barlow, P. W., 1982: The co-ordination of cell division and elongation: the role of the cytoskeleton. In: The cytoskeleton in plant growth and development (Lloyd, C. W., ed.), pp. 203–228. London: Academic Press.
Molé-Bajer, J., Bajer, A. S., 1983: Action of taxol on mitosis: modification of microtubule arrangements and function of the mitotic spindle inHaemanthus endosperm. J. Cell Biol.96, 527–540.
Packard, M. J., Stack, S. M., 1976: The preprophase band: possible involvement in the formation of the cell wall. J. Cell Sci.22, 403–411.
Pickett-Heaps, J. D., Northcote, D. H., 1966: Cell division in the formation of the stomatal complex of the young leaves of wheat. J. Cell Sci.1, 121–128.
—, 1969: Preprophase microtubules and stomatal differentiation inCommelina cyanea. Aust. J. biol. Sci.22, 375–391.
—, 1974: Plant microtubules. In: Dynamic aspects of plant ultrastructure (Robards, A. W., ed.), pp. 219–255. Maidenhead: McGraw-Hill.
Raggio, N., Raggio, M., 1956: Relación entre cotiledones y nodulación y factores que la afectan. Phyton7, 103–119.
Schmiedel, G., Schnepf, E., 1979: Side branch formation and orientation in caulonema of the moss,Funaria hygrometrica: experiments with inhibitors and with centrifugation. Protoplasma101, 47–59.
—,Reiss, H. D., Schnepf, E., 1981: Associations between membranes and microtubules during mitosis and cytokinesis in caulonema tip cells of the mossFunaria hygrometrica. Protoplasma108, 173–190.
Schmit, A. C., Vantard, M., Mey, J. de, Lambert, A. M., 1983: Aster-like microtubule centers establish spindle polarity during interphase-mitosis transition in higher plant cell. Plant Cell Reports2, 285–288.
Schnepf, E., 1981: Polarity and gradients in tip growing plant cells. In: International cell biology 1980/1981 (Schweiger, H. G., ed.), pp. 485–488. Berlin-Heidelberg-New York: Springer.
Singh, A. P., 1977: The subcellular organization of stomatal initials in sugarcane leaves: the guard and subsidiary mother cells. Can. J. Bot.55, 2801–2809.
Sinnott, E. W., Bloch, R., 1941: Division in vacuolate plant cells. Amer. J. Bot.28, 225–232.
Tiwari, S. C., Wick, S. M., Williamson, R. E., Gunning, B. E. S., 1984: Cytoskeleton and integration of cellular function in cells of higher plants. J. Cell Biol.99, 63–69.
Venverloo, C. J., Hovenkamp, P. H., Weeda, A. J., Libbenga, K. R., 1980: Cell division inNautilocalyx explants I. Phragmosome, preprophase band and plane of division. Z. Pflanzenphysiol.100, 161–174.
Wick, S. M., Duniec, J., 1983: Immunofluorescence microscopy of tubulin and microtubule arrays in plant cells. I. Preprophase band development and concomitant appearance of nuclear envelope-associated tubulin. J. Cell Biol.97, 235–243.
— —, 1984: Immunofluorescence microscopy of tubulin and microtubule arrays in plant cells. II. Transition between the preprophase band and the mitotic spindle. Protoplasma122, 45–55.
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Bakhuizen, R., van Spronsen, P.C., Sluiman-den Hertog, F.A.J. et al. Nuclear envelope radiating microtubules in plant cells during interphase mitosis transition. Protoplasma 128, 43–51 (1985). https://doi.org/10.1007/BF01273234
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DOI: https://doi.org/10.1007/BF01273234