Zusammenfassung
Die quantitative In-situ-Analyse von Dinitrophenyl-(DNP-)Aminosäuren mit Hilfe der Fluoreszenzlöschung auf Kieselgelschichten wird beschrieben. Die Dünnschichtchromatogramme mit den aufgetrennten DNP-Aminosäuren wurden mit einem Turner Fluorimeter, Modell 111, ausgerüstet mit einer Camag Dünnschichtchromatographie-Meßtür und einem 10-MV Heathkit Recorder, ausgewertet. Der Einfluß von Zeit,
Summary
The quantitative in situ determination of dinitrophenyl-(DNP-) amino acids by fluorescence quenching on silicagel layers is described. The thin-layer chromatograms on which DNP-amino acids are separated were evaluated with a Turner model 111 fluorometer equipped with a Camag TLC scanner and attached to a 10-mv Heathkit recorder. Parameters such as time, environment and direction of scanning were investigated and optimum conditions determined. The standard deviations for the reproducibility of the technique range from 3 to 9% for 6 amino acids separated in one dimension and from 7 to 13% after twodimensional chromatography. Linear relationships are found between peak areas and logarithm or square root of amino acid concentration. The direct dinitrophenylation of amino acids on the chromatogram prior to development was also investigated and shows some promise.
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Post doctoral fellow 1968. Derzeit: Forschungsabteilung der Robapharm A. G-., Basel, Schweiz.
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Borko, J., Frei, R.W. Quantitative In-situ-Fluorimetrie von Dinitrophenylaminosäuren auf Dünnschichtchromatogrammen. Mikrochim Acta 57, 1144–1149 (1969). https://doi.org/10.1007/BF01271381
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DOI: https://doi.org/10.1007/BF01271381