Summary
Nishigahara-O30M (NOPM) strain of rabies virus, which had been produced by passing the rabbit-fixed Nishigahara strain 30 times consecutively in 1-day eggs and then transferring it to the mouse brain passage, was used to prepare the 1-day egg vaccine employing beta-propiolactone inactivation. The vaccines produced exhibited satisfactory potencies in mouse protection tests, Habel indices being consistently higher than 1,000. Preservation of the vaccine in an ice box for 4 months did not reduce the potency. A standard method of vaccine preparation suited for mass-production was also worked out. A pool of 3 lots of this vaccine was given to 30 volunteers in 3 or 4 doses of 0.1 ml. by the intradermal route with intervals of 3 to 7 days, and all the vaccinees responded to the vaccination with fairly good rises of neutralizing antibodies occurring before the end of the second week, except one person who showed only a very weak response. Little systemic reactions were observed, and local reactions appeared as erythema and induration lasting for a few days, which nevertheless became less intense upon continuation of the vaccination. It was estimated therefore that the NOPM-1-day egg vaccine might be able to replace the brain type vaccine currently used for human prophylaxis.
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The present work has been supported by Grant E-4248 of the National Institutes of Health, U. S. A., Grant GA MNS 61168 of the Rockefeller Foundation, U. S. A., a grant supplied from the World Health Organization and a grant from the Japanese Ministry of Education.
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Yoshino, K., Saito, K. Infection of the one-day old fertile hen's egg with rabies virus. Archiv f Virusforschung 13, 489–498 (1963). https://doi.org/10.1007/BF01267792
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DOI: https://doi.org/10.1007/BF01267792