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Simplified fluorimetric methods for determination of vitamin B6 in food materials

Vereinfachte fluorimetrische Methoden zur Vitamin-B6-Bestimmung in Lebensmitteln

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Zusammenfassung

Eine einfache fluorometrische Methode zur Vitamin-B6-Bestimmung wurde vorgeschlagen, die den gesamten Vitamin-B6-Gehalt nach chromatographischer Beseitigung der interferierenden Substanzen auf Dowex-50-WX8-Säulen ermittelt. Pyridoxol, Pyridoxal und Pyridoxamin werden in das stark fluorescierende Lacton der 4-Pyridoxinsäure überführt. Die Methode ist spezifisch und stimmt gut mit dem mikrobiologischen Test überein. Drei bis vier Bestimmungen können in 8 Std durchgeführt werden.

Summary

A simple fluorimetric method for the determination of vitamin B6 in food materials is proposed, enabling total vitamin B6 to be determined after the chromatographic removal of interfering substances on Dowex 50 WX 8 columns. Pyridoxol, pyridoxal and pyridoxamine are transferred into the highly fluorescent lactone of 4-pyridoxic acid. The method is specific and agrees well with the microbilogical assay. Three or four determinations can be performed in eight hours.

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Authors and Affiliations

  1. Research Insitute of Food Industry, Na bělidle 21, 5, Prague

    Vlasta Fiedlerová

  2. Institute of Chemical Technology, Suchbätarova 1905, 6, Prague, Czechoslovakia

    Jiří Davídek

Authors
  1. Vlasta Fiedlerová
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  2. Jiří Davídek
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Fiedlerová, V., Davídek, J. Simplified fluorimetric methods for determination of vitamin B6 in food materials. Z Lebensm Unters Forch 166, 93–95 (1978). https://doi.org/10.1007/BF01267784

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  • DOI: https://doi.org/10.1007/BF01267784

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