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Untersuchungen zur Kristallisation von Hexonen der Adenoviren der Gruppe III

Studies on the cristallization of hexons from Adenoviruses of group III

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Zusammenfassung

Es konnte gezeigt werden, daß die Hexone der Adenovirustypen 1, 2, 5 und 6 unmittelbar aus ungereinigten Antigenkonzentraten bei pH 4,6 zur Kristallisation gebracht werden können. Die Zentrifugation der Kristalle in einer Saccharoselösung diente der Abtrennung von der Hauptmenge der Begleitproteine. Mehrere Rekristallisationen führten zu hochgereinigten Präparaten. Die Bedingungen der Kristallisation wurden untersucht. Die Kristallform zeigte Veränderungen in Abhängigkeit von den Reaktionsbedingungen. Die Hexonkristalle der einzelnen Virustypen ließen sich morphologisch nicht unterscheiden.

Summary

It proved possible to crystallize the hexons of Adenovirus types 1, 2, 5, and 6 directly from concentrated antigensuspensions at pH 4.6. The crystals could then be separated from the majority of contaminating proteins by sedimentation into sucrose solutions. Finally, multiple recrystallization of the hexons resulted in highly pure preparations. The shape of the crystals depended largly on the conditions of crystallization. However, crystals derived from hexons of individual Adenovirus types were indistinguishable.

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  1. L. Döhner &  H. Hudemann

    Present address: Institut für Medizinische Mikrobiologie und Epidemiologie der Medizinischen Akademie Magdeburg, DDR

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    1. L. Döhner
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    2. H. Hudemann
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    Döhner, L., Hudemann, H. Untersuchungen zur Kristallisation von Hexonen der Adenoviren der Gruppe III. Archiv f Virusforschung 38, 279–289 (1972). https://doi.org/10.1007/BF01262818

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    • DOI: https://doi.org/10.1007/BF01262818

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