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Serial analysis of microtubules in cultured rat sensory axons

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Journal of Neurocytology

Summary

A fascicle of axons in an organotypic culture of rat dorsal root ganglion was analysed in 190 serial transverse sections, a total fascicle length calculated to be 12.8μm. The number, position and continuity of microtubules and components of smooth endoplasmic reticulum (SER) were examined. The 187 microtubules traced in 27 axons showed a total of 32 terminations in the series; 14 of these were ‘starts’ and 18 were ‘stops’ in relation to progression away from the neuronal soma; three tubules began and ended in the series. Microtubules overall were long, their lengths calculated to average 108μm. Terminations usually occurred close to the plasma membrane but otherwise exhibited no obvious morphological specialization. Microtubules in some axons were concentrated in the periphery of the axon, a region from which neurofilaments were excluded. The relative position of tubules in the axon did not change in any systematic way (for example, by rotation) throughout the series. The SER showed marked variation in form, from the expected membrane-bounded vesicle to profiles that resembled a microtubule or a thinner densely stained filament.

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Author notes

  1. Drnnis Bray

    Present address: MRC Cell Biophysics Unit, 26 Drury Lane, WC2B 5RL, London, UK

Authors and Affiliations

  1. Department of Anatomy and Neurobiology, Washington University School of Medicine, 660 South Euclid Avenue, 63110, St Louis, MO, USA

    Drnnis Bray & Mary Bartlett Bunge

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  1. Drnnis Bray
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  2. Mary Bartlett Bunge
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Bray, D., Bunge, M.B. Serial analysis of microtubules in cultured rat sensory axons. J Neurocytol 10, 589–605 (1981). https://doi.org/10.1007/BF01262592

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  • DOI: https://doi.org/10.1007/BF01262592

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