Summary
Investigations have suggested that lysozyme (E.C. 3.2.1.17.) is involved in bone mineralization. High concentrations of lysozyme is found in the growth plate near cartilage bone junction, where it is located at the collagen fibrils and in the ground substance.
Quantitative studies of lysozyme levels were made in ossifying tissue of healing fractures, to confirm the existence of this relationship on bone repair. Callous tissue, serum samples and normal bone was collected from 42 rats at 15 intervalls during a 50 day healing period.
Agar gel diffusion test was used for quantitation of lysozyme. Electrophoresis of tissue extract and standard henn egg white lysozyme served as control. Lysozyme levels in callous tissue increased significantly (4- 5-fold) from 4. - 21. day p. trauma and subsequently decreased.
The concentration in serum samples did not change significantly. Changes in Ca concentration and histological studies during tests confirm a direct relationship between bone mineralization and lysozyme level changes.
Zusammenfassung
An 42 Ratten wurde der Lysozymgehalt von Callus- und Knochengewebe im Verlaufe der unbeeinflußten Frakturheilung mit der Agardiffusionsmethode nach Ossermann bestimmt.
Der Lysozymgehalt vom Callusgewebe steigt vom 4.- 21. Tag nach Fraktursetzung auf das 4fache an und fällt bis zum 50. Tag wieder auf Normalwerte ab. Der Calciumgehalt von Callusgewebe steigt beginnend am 10. Tag nach Fraktursetzung kontinuierlich bis zum 50. Tag an.
Diese Ergebnisse lassen erkennen, daß Lysozym bei der Knochenneubildung aus Callusgewebe beteiligt ist. Mögliche Reaktionen von Lysozymen mit Bestandteilen der nicht verkalkten Knochenmatrix werden diskutiert. Lysozym scheint durch Disaggregation der Proteoglykane diese zu inaktivieren, wodurch die Nukleation und Einlagerung von Hydroxylapatit an Kollagenfibrillen in Gang gesetzt wird.
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Siebert, H., Treber, N., Konold, P. et al. Zur Rolle des Lysozyms bei der Mineralisation von Callusgewebe. Langenbecks Arch Chiv 346, 193–199 (1978). https://doi.org/10.1007/BF01261242
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DOI: https://doi.org/10.1007/BF01261242