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The intracellular organization of actin and tubulin in cultured C-1300 mouse neuroblastoma cells (clone NB41A3)

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Journal of Neurocytology

Summary

Cells of clone NB41A3 of the C-1300 mouse neuroblastoma were grown to a critical density at which many of the cells flatten, assume a variety of shapes and sizes and some sprout processes resembling neurites. We have studied the distribution of actin and tubulin in these cells using fluorescence microscopy and antibodies against actin or tubulin under these conditions. Actin-containing structures are variably arranged and predominantly associated with motile areas of the cell periphery including the growth cone. Microtubules appear to run radially from the perinuclear area towards the cell periphery. When neurites are present, microtubules converge into them and run to the growth cone but rarely contact its edge.

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Authors and Affiliations

  1. Department of Human Anatomy, University of Turin, School of Medicine, Turin, Italy

    Pier Carlo Marchisio

  2. Max Planck Institute for Biophysical Chemistry, Goettingen, West Germany

    Mary Osborn & Klaus Weber

Authors
  1. Pier Carlo Marchisio
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  2. Mary Osborn
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  3. Klaus Weber
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Marchisio, P.C., Osborn, M. & Weber, K. The intracellular organization of actin and tubulin in cultured C-1300 mouse neuroblastoma cells (clone NB41A3). J Neurocytol 7, 571–582 (1978). https://doi.org/10.1007/BF01260890

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  • DOI: https://doi.org/10.1007/BF01260890

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