Examination of fungal growth and aflatoxin production on marihuana


Under favorable growth conditions,Aspergillus flavus andA. parasiticus produced aflatoxins on marihuana. Cultures ofA. flavus ATCC 15548 produced both aflat oxin B1(AFB1) and G1(AFG1). The production of AFG1 was substantially greater than that of AFB1. Cultures ofA. flavus NRRL 3251 andA. parasiticus NRRL 2999 produced only AFB1. All natural flora cultures tested negative for aflatoxins. NoAspergilli sporulations were observed in these cultures. In the cultures inoculated with known toxigenic fungi, the highest mean level for total aflatoxins was 8.7 μg/g of medium. Marihuana appears not to yield large quantities of these mycotoxins but sufficient levels are present to be a potential health hazard for both the user and the forensic analyst who is in daily contact with such plant material. Careful processing, storage, and sanitation procedures should be maintained with marihuana. If these conditions are disregarded due to the illicit status of marihuana, the potential for mycotoxin contamination must be considered.

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  1. 1.

    Abelson, H. & R. B. Atkinson. 1975. Public Experience with Psychoactive Substances, pp. 1–24. Response Analysis Corps, Princeton, N.J.

    Google Scholar 

  2. 2.

    Arseculeratne, S. N., L. M. Desilva, S. Wisesundera & C. H. S. R. Bandunatha. 1969. Coconut as a medium for experimental production of aflatoxin. Appl. Microbiol. 18: 88–94.

    Google Scholar 

  3. 3.

    Butler, W. H. 1969. Aflatoxicosis in laborabory animals, p. 223–236. In: Aflatoxin: Scientific Background, Control, and Implication. (ed.) L. A. Goldblatt, Academic Press, New York.

    Google Scholar 

  4. 4.

    Butler, W. H. 1974. Aflatoxin, pp. 1–25. In: Mycotoxins, (ed.) I. F. H. Purchase, Elsevier Pub. Co., Amsterdam.

    Google Scholar 

  5. 5.

    Davis, N. D. & U. L. Diener. 1968. Growth and aflatoxin production by Aspergillus parasiticus from various carbon sources. Appl. Microbiol. 16: 158–159.

    Google Scholar 

  6. 6.

    Diener, U. L. & N. D. Davis. 1969. Aflatoxin formation by Aspergillus flavus, pp. 13–54. In: Aflatoxin: Scientific Background, Control and Implication. (ed.) L. A. Goldblatt, Academic Press, New York.

    Google Scholar 

  7. 7.

    Golumbic, C. & M. Kulik. 1969. Fungal spoilage on stored crops and its control, pp. 307–333. In: Aflatoxin: Scientific Background, Control and Implication. (ed.) L. A. Goldblatt, Academic Press, New York.

    Google Scholar 

  8. 8.

    Gupta, S. K. & T. A. Venkitasubramanian. 1975. The effect of zinc and phytic acid on the incorporation of 1 —14C-acetate into aflatoxin by resting mycelia of Aspergillus parasiticus. Z. Lebonsm. Onters. Forch 159: 107–111.

    Google Scholar 

  9. 9.

    Hilldrup, J. L., T. Eadie & G. C. Llewellyn. 1977. Fungal groth and aflatoxin production on apiarian substrates. J. Assoc. Offic. Anal. Chemists. 60: 96–99.

    Google Scholar 

  10. 10.

    Horwitz, W., A. Senze & H. Reynolds. 1975. Natural poisons pp. 465. In: Official Methods of Analysis. Assoc. Offic Anal. Chemists Soc. Washington, D.C.

    Google Scholar 

  11. 11.

    Lee, E. G. H., P. M. Townsley & C. C. Walden. 1966. Effects of bivalent metals on the production of aflatoxins is submerged cultures. J. Food Sci. 31: 432–435.

    Google Scholar 

  12. 12.

    Lin, M. T. & J. C. Dianese. 1976. A coconut-agar medium for rapid detection of aflatoxin production by Aspergillus spp. Phytopathology. 66: 1466–1469.

    Google Scholar 

  13. 13.

    Llewellyn, G. C., W. W. Carlton, J. E. Robbers & W. G. Hansen. A rapid and simplified method for the preparation of pure aflatoxin B1. Dev. Ind. Microbiol. 14: 325–330.

  14. 14.

    Llewellyn, G. C. & T. Eadie. 1974. Evaluating the potential for occurrence of aflatoxins on black sunflower seeds. J. Assoc. Offic. Anal. Chemists. 57: 858–860.

    Google Scholar 

  15. 15.

    Lo, W. B. & H. S. Black. 1972. Effects of aflatoxin B1 on human skin lipids metabolism. site of action on [1-14C] — acetate incorporation. Experientia 28: 1278–1279.

    Google Scholar 

  16. 16.

    Maggon, K. K., S. Gopal & T. A. Venkitasurbamanian. 1974. Metabolism of Aspergillus flavus and A. parasiticus with special reference to factors affecting aflatoxin formation. Biochem. Physiol. Pflanzen. 166: 327–331.

    Google Scholar 

  17. 17.

    Pattee, H. E. 1969. Production of aflatoxins by Aspergillus flavus cultured on flue-cured tobacco. Appl. Microbiol. 18: 952–953.

    Google Scholar 

  18. 18.

    Sheretz, P. C., T. Eadie, J. W. Young & G. C. Llewellyn. 1976. Aflatoxin occurrence on raw and cooked york soybeans inoculated with three Aspergilli isolates. J. Assoc. Offic. Anal. Chemists. 59: 662–665.

    Google Scholar 

  19. 19.

    Shotwell, O. K., R. D. Stubblefield, C. W. Hesseltine & W. G. Sorenson. 1966. Production of aflatoxin on rice. Appl. Microbiol. 14: 425–432.

    Google Scholar 

  20. 20.

    Smith, J. K. & D. B. Louria. 1974. Isolation of aflatoxinrelated substance from tobacco. Arch. Environ. Health 28: 170–171.

    Google Scholar 

  21. 21.

    Swaminathan, B. & P. E. Koehler. 1976. Isolation of an inhibitor of Aspergillus parasiticus from white potatoes (Solanum tuberosum). J. Food Sci. 41: 313–319.

    Google Scholar 

  22. 22.

    Tso, T. C. & T. D. Sorokin. 1967. Examination of aflatoxin B1 in leaf tobacco and in cigarette smoke condensate. Beitr. Tabarforsch. 1: 18–20.

    Google Scholar 

  23. 23.

    Turner, C. D. & K. Hadley. 1973. Constituents of Cannabis sativa L. II: Absence of cannabidiol in Africa variant. J. Phar. Sci. 62: 251–255.

    Google Scholar 

  24. 24.

    Welty, R. E. & G. B. Lucas. 1969. Fungi isolated from flue-cured tobacco. Appl. Microbiol. 16: 851–854.

    Google Scholar 

  25. 25.

    Welty, R. E., G. B. Lucas, J. T. Fletcher & H. Yang. 1968. Fungi isolated from tobacco leaves and brownspot lesions before and after flue-curing. Appl. Microbiol. 16: 1309–1313.

    Google Scholar 

  26. 26.

    Welty, R. E. & G. B. Lucas. 1969. Fungi isolated from flue-cured tobacco at time of sale and after storage. Appl. Microbiol. 17: 360–365.

    Google Scholar 

  27. 27.

    Wogan, G. N. 1968. Biochemical responses to aflatoxins. Cancer Res. 28: 2282–2287.

    Google Scholar 

  28. 28.

    Wogan, G. N. & P. M. Newberne. 1967. Dose-response characteristics of aflatoxin B1 carcinogenesis in the rat. Cancer Res. 27: 2370–2376.

    Google Scholar 

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Llewellyn, G.C., O'Rear, C.E. Examination of fungal growth and aflatoxin production on marihuana. Mycopathologia 62, 109–112 (1977). https://doi.org/10.1007/BF01259400

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  • Aspergillus
  • Aflatoxin
  • Fungal Growth
  • Careful Processing
  • Sufficient Level