Summary
Because it is not possible to use donor specific antigens for the induction of immunological enhancement in cadaveric organtransplantation, attempts were made to use polyspecific antigens in the enhancement of orthotopic canine liver allotransplants. Of 34 mongrel recipients, 17 controls survived for 6.9 ± 1.5 days. Six recipients (group 3) were given 750 mg/kg polyspecific, semisoluble antigen prepared from 20 spleens (PSEA 20) together with 10 mg/kg prednisolone on days 15, 8 and 1 before transplantation. The mean survival time of this group was 10.1 ± 2.0 days (P < 0.01 compared with the control group). Six other recipients (group 4) were treated similiarly, except that the antigen had been prepared from a pool of 70 spleens (PSEA 70). Survival was variable here: 3 survived for more than 3 weeks and the other 3 died on days 1, 7 and 8 postoperatively, with signs of accelerated rejection.
Donors and recipients were not identical for LD determinants, but one donor recipient pair with near identity showed a higher degree of enhancement. The recipients displaying accelerated rejection had markedly higher lymphocytotoxic and haemagglutinating antibodies. Animals surviving for longer periods had low antibody titres. In addition, all recipients progressive rejection were found to show inhibition of leucocyte migration. After three antigen doses rosette-forming lymphocytes were present in increased numbers in peripheral blood, and remained unchanged thereafter.
Zusammenfassung
Aktives Enhancement mit spenderspezifischem Antigen ist bei der klinischen Kadaver-Organtransplantation nicht praktikabel. Deshalb wurde der Versuch gemacht, ein aktives Enhancement von orthotopen Hundeleberallotransplantaten mit polyspezifischem Antigen zu erzielen. Die 17 Tiere der Kontrollgruppe überlebten durchschnittlich 6,9 ± 1,5 Tage. 6 Empfänger (Gruppe 3) erhielten 750 mg/kg polyspezifisches halblösliches (PSEA 20) Antigen, welches aus 20 Milzen hergestellt wurde, zusammen mit 10 mg/kg Prednisolon 15, 8 und 1 Tag vor der Transplantation. Die durchschnittliche Überlebensdauer in dieser Gruppe betrug 10,1 ± 2,0 Tage (P < 0,01, gegen die Kontrollgruppe). Weitere 6 Hunde (Gruppe 4) wurden wie die Tiere de Gruppe 3 vorbehandelt; jedoch wurde das Antigen hier aus 70 Nilzen hergestellt (PSEA 70). Die Überlebensdauer war sehr unterschiedlich: 3 Tiere überlebten mehr als 3 Wochen, die anderen 3 starben 1, 7 und 8 Tage nach der Transplantation unter Zeichen der beschleunigten Abstoßung. Spender und Empfänger waren in keinem Fall LD-identisch, aber ein nahezu identisches Spender-Empfänger-Paar zeigte einen besseren Enhancement-Effekt. Die Empfänger mit der beschleunigten Abstoßung zeigten ungewöhnlich hohe Lymphocytotoxin-und Hämagglutinin-Präsenz, länger überlebende Tiere niedrige Antikörper-Titer. Bei allen Empfängern mit starker Rejektion war die Leukocyten-Migration inhibiert. Rosetten-bildende Lymphocyten waren nach dreimaliger Antigenapplikation vermehrt im Blut nachweisbar und blieben danach konstant.
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Lie, T.S., Holst, A., Kanda, M. et al. Aktives Enhancement von Hundeleberallotransplantaten Vorbehandlung mit polyspezifischem semisolublen Milzalloantigen. Langenbecks Arch Chiv 344, 15–26 (1977). https://doi.org/10.1007/BF01259349
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DOI: https://doi.org/10.1007/BF01259349