Abstract
Given a uniform N source, the δ15N of barley shoots provided a genotypic range within treatments and a separation between control and salt-stress treatments as great as did δ13C*. Plant δ15N has been represented in the literature as a bioassay of external source δ15N and used to infer soil N sources, thus precluding consideration of the plant as a major cause in determining its own 815N. We believe this to be the first report of plant δ15N as a genetic trait. No mechanistic model is needed for use of δ15N as a trait in controlled studies; however, a qualitative model is suggested for further testing.
Abbreviations
- δ15N (or β13C):
-
the difference between: (1) the ratio of heavy to light isotopes of the element in a sample and (2) that of its reference standard
References
Dawson IK, Chalmers KJ, Waugh R, Forster BP, Hall J, Powell W (1993) Detection of genetic variation in Hordeum spontaneum populations from Israel using RAPD markers. Mol Ecol 2: 151–159
Domenach AM, Kurdali F, Bardin R (1989) Estimation of symbiotic dinitrogen fixation in alder forest by the method based on natural15N abundance. Plant Soil 118: 51–59
Epstein E (1994) The anomaly of silicon in plant biology. Proc Natl Acad Sci USA 91: 11–17
Handley LL, Raven JA (1992) The use of natural abundance of nitrogen isotopes in plant physiology and ecology. Plant Cell Environ 15: 965–985
Handley LL, Daft MJ, Wilson J, Scrimgeour CM, Ingleby K, Sattar MA (1993) Effects of the ecto- and VA-mycorrhizal fungiHydnagium carneum andGlomus clarum on the δ15N and δ13C values ofEcucalyptus globulus andRicinus communis. Plant Cell Environ 16: 375–382
Handley LL, Nevo E, Raven JA, Martinez-Carrasco R, Scrimgeour CM Pakniyat H, Forster BP (1994) Chromosome 4 controls potential water use efficiency (δ13C) in barley. J Exp Bot 45: 1661–1663
Handley LL, Scrimgeour CM (1997) Terrestrial plant ecology and15N natural abundance: The present limits to interpretation for uncultivated systems with original data from a Scottish old field. Adv Ecol Res, in press
Hewitt EJ (1966) Sand and water culture methods used in the study of plant nutrition, 2nd Edn. Commonwealth Agricultural Bureaux. Farnham Royal, UK
Kohls SJ, van Kessel C, Baker DD, Grigal DF, Lawrence DB (1994) Assessment of N2 fixation and N cycling byDryas along a chronosequence within the forelands of the Athabasca Glacier, Canada. Soil Biol Biochem 26: 623–632
Kohorn LU, Goldstein G, Rundel PW (1994) Morphological and isotopic indicators of growth environment: variability in δ13C in Simmondsia chinensis, a dioecious desert shrub. J Exp Bot 45: 1817–1822
Mariotti A, Mariotti F, Amarger N, Pizelle G, Ngambi, J-M, Champigny M-L, Moyse A (1980) Fractionnements isotopiques de l'azote lors de processus d'absorption des nitrates et de fixation de l'azote atmosphérique par les plantes. Physiol Veg 18: 163–181
Mariotti A, Martiotti F, Champigny M-L, Amarger N, Moyse A (1982) Nitrogen isotope fractionation associated with nitrate reductase activity and uptake of nitrate by pearl milletPennisetum spp. Plant Physiol 69: 880–884
Michelsen A, Schmidt IK, Jonasson S, Quarmby C. Sleep, D (1996)15N abundance of subarctic plants provides field evidence that ericoid, ectomycorrhizal and non-arbuscular mycorrhizal species access different sources of soil nitrogen. Oecologia 105: 53–63
O'Leary M (1988) Carbon isotopes in photosynthesis. BioScience 38: 328–336
Vitousek PM, Shearer G, Kohl DH (1989) Foliar15N natural abundances in Hawaiian rainforest: patterns and possible mechanisms. Oecologia 78: 383–388
Winer BJ (1962) Statistical principles in experimental design. McGraw-Hill. New York
Yoneyama T (1995) Nitrogen metabolism and fractionation of nitrogen isotopes in plants. In: Wada E, Yoneyama T, Minagawa M, Ando T, Fry BD (eds) Stable isotopes in the biosphere. Kyoto University Press, Kyoto, pp 92–102
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Handley, L.L., Robinson, D., Forster, B.P. et al. Shoot δ15N correlates with genotype and salt stress in barley. Planta 201, 100–102 (1997). https://doi.org/10.1007/BF01258686
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DOI: https://doi.org/10.1007/BF01258686