Abstract
The wild-type and three deletional interposon mutants (psaE, ndhF, psaE/ndhF) ofSynechococcus sp. PCC7002 were analysed with respect to their ability to induce the CO2-concentrating mechanism (CCM). The response of thendhF mutant was similar to that of the control with normal adaptation of CO2 and HCO3 −3 transport at low CO2. In contrast, thepsaE mutant was unable to grow normally at 20 μL·L−1 and could not induce high-affinity HCO −3 uptake. The double mutant showed an even greater degree of impaired growth than thepsaE mutant, even at 350 μL·L− CO2. It also showed a relatively specific defect in the ability of the cells to induce high-affinity HCO −3 -uptake, although when growth was severely restricted at 20 μL·L−1 CO2 there may also have been some effect on the induction of CO2 transport. Our results indicate a key role for psaE-mediated, and to a lesser extent ndhF-mediated, electron transport in efficient utilisation of HCO −3 under low inorganic carbon inSynechococcus sp. PCC7002.
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Abbreviations
- BTP:
-
Bis-Tris-propane
- CCM:
-
CO2-concentrating mechanism
- Ci:
-
inorganic carbon (CO2 + HCO −3 )
- high-Ci cells:
-
cells grown on 2% CO2
- K1/2 :
-
substrate concentration required for 50% of maximum activity
- low-Ci cells:
-
cells grown on 20 μL·L−1 CO2
- NDH-1:
-
NAD(P)H dehydrogenase complex 1
- Rubisco:
-
ribulose-1,5-bisphosphate carboxylase/oxygenase
- V max :
-
maximum rate
- wt:
-
wild type
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Sültemeyer, D., Dean Price, G., Bryant, D.A. et al. PsaE- and NdhF-mediated electron transport affect bicarbonate transport rather than carbon dioxide uptake in the cyanobacteriumSynechococcus sp. PCC7002. Planta 201, 36–42 (1997). https://doi.org/10.1007/BF01258678
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DOI: https://doi.org/10.1007/BF01258678