Skip to main content
SpringerLink
Log in

Time relationships in the development of cytopathic response to vaccinia virus in tissue culture tubes

  • Published:
Archiv für die gesamte Virusforschung Aims and scope Submit manuscript

Summary

The time of appearance of cytopathic changes was studied in vaccinia virus infected tissue culture tubes. From proportions of consecutively recorded positive tubes it was inferred that the cumulative percentages of newly formed plaques plotted on a probit scale formed a straight line against time. In a given experiment this distribution was of the same type irrespective of the virus dose.

In repeatedly read virus titrations an approximately linear relationship was found to exist between the logarithm of virus dilution and the reciprocal of incubation time (= the period from the moment of inoculation until the first positive reading). The relation was rectilinear within a dose interval of 4–5 log units but outside this range departure from linearity was found.

For practical reasons the incubation time method was less useful as a routine procedure for virus assay.

End-point titers in monkey kidney tubes increased by 0.15–0.20 log from day 6 to day 12.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Meynell, G. G., andE. W. Meynell: The growth of microorganisms in vivo with particular reference to the relation between dose and latent period. J. Hyg. (London)56, 323–346 (1958).

    Google Scholar 

  2. Kjellén, L.: Studies on Adenoviruses (APC-RI-ARD) in tissue culture. Correlation between the amount of virus inoculated and the time needed for production of cellular degeneration. Arch. ges. Virusforsch.7, 110–119 (1956).

    PubMed  Google Scholar 

  3. Tigertt, W. D., A. S. Benenson, andW. S. Gochenour: Airborne Q fever. Bact. Rev.25, 285–293 (1961).

    PubMed  Google Scholar 

  4. Gard, S.: Encephalomyelitis of mice. II. A method for the measurement of virus activity. J. exp. Med.72, 69–77 (1940).

    Google Scholar 

  5. Gordon Smith, C. E., andD. R. Westgarth: The use of survival time in the analysis of neutralization tests for serum antibody surveys. J. Hyg. (London)55, 224–238 (1957).

    Google Scholar 

  6. Brownlee, K. A., andD. Hamre: Studies on chemotherapy of vaccinia virus. I. An experimental design for testing antiviral agents. J. Bact.61, 127–134 (1951).

    PubMed  Google Scholar 

  7. Bauer, D. J., K. H. Dumbell, P. Fox-Hulme, andP. W. Sadler: The chemotherapy of variola major infections. Bull. WHO26, 727–732 (1962).

    PubMed  Google Scholar 

  8. Helso- och sjukvården. Medicinalstyrelsens underdåniga berättelse för år 1884, p. 62. Bidrag till Sveriges officiella Statistik, P. A. Norstedt & Söner, Stockholm, 1886.

  9. Melnick, J. L., C. Rappaport, D. D. Banker, andP. N. Bhatt: Stabilized suspensions of monkey kidney cells suitable for intercontinental shipment. Proc. Soc. exp. Biol.88, 676–678 (1955).

    Google Scholar 

  10. Finney, D. J.: Probit Analysis. Cambridge University Press, 1952.

  11. Snedecor G. W.: Statistical Methods. The Iowa State College Press, Ames, Iowa, U.S.A., 1956.

    Google Scholar 

  12. Kärber, G.: Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Arch. exp. Path. Pharmakol.162, 480–487 (1931).

    Google Scholar 

  13. Espmark, J. Å.: Tissue culture end-point titrations as a routine potency test for smallpox vaccine. Arch. ges. Virusforsch.15, 35–49 (1964).

    PubMed  Google Scholar 

  14. Bryan, W. R., andJ. W. Beard: Estimation of purified papilloma virus protein by infectivity measurements. J. infect. Dis.65, 306–321 (1939).

    Google Scholar 

  15. Golub, O. J.: A single-dilution method for the estimation of LD50 titres of the Psittacosis-LGV group of viruses in chick embryos. J. Immunol.59, 71–82 (1948).

    Google Scholar 

  16. Cooper, P. D.: The plaque assay of animal viruses. Advances in Virus Research.8, 319–378 (1961).

    PubMed  Google Scholar 

  17. Lindenmann, J., andG. E. Gifford: Studies on vaccinia virus plaque formation and its inhibition by interferon. I. Dynamics of plaque formation by vaccinia virus. Virology19, 283–293 (1963).

    PubMed  Google Scholar 

  18. Böttiger M., E. Lycke, B. Melén, andG. Wrange: Inactivation of poliomyelitis virus by formaldehyde. Arch. ges. Virusforsch.8, 260–267 (1958).

    Google Scholar 

  19. Kjellén L.: A study of Adenovirus-host cell system by the plaque technique. Virology14, 234–239 (1961).

    PubMed  Google Scholar 

  20. Espmark J. Å., andS. Gard: Dose-incubation time relationships in virus infected tissue cultures. Theoretical considerations. Arch. ges. Virusforsch.15, 137–150 (1964).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Bacteriological Laboratory and the Department of Virus Research, Karolinska Institutet, School of Medicine, Stockholm, Sweden

    J. Åke Espmark

Authors
  1. J. Åke Espmark
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Espmark, J.Å. Time relationships in the development of cytopathic response to vaccinia virus in tissue culture tubes. Archiv f Virusforschung 15, 123–136 (1965). https://doi.org/10.1007/BF01257724

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01257724

Keywords

Search

Navigation