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A simple procedure for the quantitative microdetermination of rhamnose

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Summary

A simple spectrophotometric procedure for microdetermination of rhamnose is described. It is based on breakdown of the sugar with sulphuric acid followed by treatment withp-hydroxydiphenyl. The coloured product is measured spectrophotometrically at 560 nm. A linear relationship exists between the amount of rhamnose (10-0μg−) and the absorbance. The method is recommended for determination of rhamnose in samples containing rhamnose, different neutral sugars, amino-sugars, sugar alcohols, uronic acids, and muramic acid.

Zusammenfassung

Ein einfaches spektralphotometrisches Verfahren zur Mikrobestimmung von Rhamnose wurde beschrieben. Es beruht auf der Spaltung des Zuckers mit Schwefelsäure und nachfolgender Behandlung mit p-Hydroxydiphenyl. Das gefärbte Reaktionsprodukt wird spektralphotometrisch bei 560 nm gemessen. Die Extinktion steht mit der Rhamnosemenge in linearer Beziehung. Das Verfahren wird für die Rhamnosebestimmung in Proben empfohlen, die verschiedene Neutralzucker, Aminozucker, Zuckeralkohole, Uronsäuren und Muramsäure enthalten.

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Authors and Affiliations

  1. “Ruder Boskovic” Institute, Zagreb, Yugoslavia

    Olga Hadžija & Maja Tonković

Authors
  1. Olga Hadžija
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  2. Maja Tonković
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Hadžija, O., Tonković, M. A simple procedure for the quantitative microdetermination of rhamnose. Mikrochim Acta 66, 49–53 (1976). https://doi.org/10.1007/BF01257092

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  • DOI: https://doi.org/10.1007/BF01257092

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