Skip to main content
SpringerLink
Log in

The occurrence of glycogen-membrane complexes in fungi an electron microscope study

  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

Membrane-particle complexes were studied in the vegetative hyphae ofSclerotinia fructigena. The established techniques of differential staining for electron microscopy and pretreatment with ribonuclease andα-amylase indicated that the particles were glycogen.

The physiological significance of such structures in fungi is unknown at present, but their resemblance to similar glycogen-membrane complexes existing in animal cells is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Andersson-Cedergren, E., andU. Muscatello, 1963: The participation of the sarcotubular system in glycogen metabolism. J. Ultrastruct. Res.8, 391–401.

    PubMed  Google Scholar 

  • Baker, R. F., 1963: On the identification of glycogen in electron micrographs. J. Histochem. Cytochem.11, 284–285.

    Google Scholar 

  • Flaks, B., 1968: Formation of membrane-glycogen arrays in rat hepatoma cells. J. Cell Biol.36, 410–414.

    PubMed  Google Scholar 

  • Garant, P. R., 1968: Glycogen-membrane complexes within mouse striated muscle cells. J. Cell Biol.36, 648–652.

    PubMed  Google Scholar 

  • Ghadially, F. N., andE. W. Parry, 1966: Ultrastructure of a human hepatocellular carcinoma and surrounding non-neoplastic liver. Cancer19, 1989–2004.

    PubMed  Google Scholar 

  • Huseman, E., undH. Ruska, 1940: Die Sichtbarmachung von Molekülen des p-Jodenbenzoilglycogens. Naturwissenschaften28, 534.

    Google Scholar 

  • Huxley, H. E., andG. Zubay, 1961: Preferential staining of nucleic acid containing structures for electron microscopy. J. Biophys. Biochem. Cytol.11, 273–296.

    PubMed  Google Scholar 

  • Jensen, W. A., 1968: Cotton embryogenesis. Polysome formation in the zygote. J. Cell Biol.36, 403–406.

    PubMed  Google Scholar 

  • Luft, J. H., 1956: Permanganate: a new fixative for electron microscopy. J. Biophys. Biochem. Cytol.2, 799–801.

    PubMed  Google Scholar 

  • , 1961: Improvements in epoxy resin embedding methods. J. Biophys. Biochem. Cytol.9, 409–414.

    PubMed  Google Scholar 

  • Revel, J. P., L. Napolitano, andD. W. Fawcett, 1960: Identification of glycogen in electron micrographs of thin tissue sections. J. Biophys. Biochem. Cytol.8, 575–589.

    PubMed  Google Scholar 

  • , 1964: Electron microscopy of glycogen. J. Histochem. Cytochem.12, 104–114.

    PubMed  Google Scholar 

  • Reynolds, E. S., 1963: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Biophys. Biochem. Cytol.17, 208–212.

    Google Scholar 

  • Sabatini, D. D., K. Bensch, andR. J. Barrnett, 1963: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol.17, 19–58.

    PubMed  Google Scholar 

  • Steiner, J. W., K. Miyai, andM. J. Phillips, 1964: Electron microscopy of membraneparticle arrays in liver cells of ethionine-intoxicated rats. Amer. J. Path.44, 169–214.

    PubMed  Google Scholar 

Download references

Author information

Author notes

  1. F. D. Calonge

    Present address: Instituto Botanico “A. J. Cavanilles” (C.S.I.C.), Madrid-14, Spain

Authors and Affiliations

  1. Department of Botany, University of Bristol, England

    F. D. Calonge

Authors
  1. F. D. Calonge
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Calonge, F.D. The occurrence of glycogen-membrane complexes in fungi an electron microscope study. Protoplasma 67, 79–85 (1969). https://doi.org/10.1007/BF01256768

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01256768

Keywords

Search

Navigation