Summary
The molecular weights of3H-L-glutamate binding in the presence of chloride and calcium ions and in the presence of sodium ions were determined by the high energy irradiation technique.
The molecular weight of sodium dependent3H-L-glutamate binding, which has pharmacological specificities similar to the high-affinity uptake system for L-glutamate, was 670,000 daltons.
The high-energy radiation inactivation study of chloride and calcium dependent and sodium independent3H-L-glutamate binding is consonant with the idea that, this binding represent glutamate transport into resealed plasma membrane vesicles.
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Honoré, T., Drejer, J., Nielsen, M. et al. Differentiation of Cl−/Ca2+-dependent and sodium dependent3H-glutamate binding to cortical membranes from rat brain by high energy radiation inactivation analysis. J. Neural Transmission 65, 93–101 (1986). https://doi.org/10.1007/BF01256485
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DOI: https://doi.org/10.1007/BF01256485