Journal of Neural Transmission

, Volume 64, Issue 3–4, pp 285–293 | Cite as

Potentiation of pancuronium induced neuromuscular blockade by calcium channel blockersin vitro

  • W. Ilias
  • K. Steinbereithner
Original Papers


The calcium channel blockers verapamil, methoxyverapamil, diltiazem, and nisoldipine have been tested for interactions with the nondepolarizing muscle relaxant pancuronium bromide by using the indirectly stimulatedin vitro preparation of the rat hemidiaphragm. Dose response curves of each calcium channel blocker, pancuronium bromide, and combinations of both were determined.

Each of the four calcium channel blocking drugs showed agonistic interaction in potentiating pancuronium induced neuromuscular blockade.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bikhazi, G. B., Thomas, K. C., Foldes, F. F.: Effects of verapamil on mammalian musclein vitro. Anesthesiology51, 3-S275 (1979).Google Scholar
  2. Colvin, R. A., Pearson, N., Messineo, F. C., Katz, A. M.: Effects of Ca-channel blockers on Ca transport and ATPase in skeletal and cardiac sarcoplasmic reticulum vesicles. J. Cardiovasc. Pharm.4, 935–941 (1982).Google Scholar
  3. Durant, N. N., Nguyen, N., Katz, R. L.: Potentiation of neuromuscular blockade by verapamil. Anesthesiology60, 298–303 (1984).PubMedGoogle Scholar
  4. Ferry, D. R., Goll, A., Glossmann, H.: Differential labelling of putative skeletal muscle calcium channels by (3H)-Nifedipine, (3H)-Nitrendipine, (3H)-Nimodipine, and (3H)-PN-200110. Nauyn-Schmiedeberg's Arch. Pharmacol.323, 276–277 (1983).Google Scholar
  5. Gonzales-Serratos, H., Valle-Aguilera, R., Lathrop, D. A., Garcia, Maria del Carmen. Nature289, 292–294 (1982).Google Scholar
  6. Gould, R. J., Murphy, K. M., Snyder, S. H.: (3H)-Nitrendipine-labelled calcium channels discriminate inorganic calcium agonists and antagonists. Proc. Natl. Acad. Sci. U.S.A.79, 3656–3660 (1982).PubMedGoogle Scholar
  7. Hoefling, B., Renner, H., Westphal, W.: Changes of neuromuscular transmission in mammalian skeletal muscle induced by Iproveratril (Verapamil). Pfluegers Arch.375, R29–113 (1976).Google Scholar
  8. Ilias, W., Lackner, F., Zekert, F.: Nifedipin and Vecuronium-bromid: wie reagiert der mit Ca-Antagonisten behandelte Patient auf nichtdepolarisierende Muskelrelaxantien. Der Anästhesist34, in press (1985).Google Scholar
  9. Ilias, W., Steinbereithner, K.: Zur Wechselwirkung der Ca-Antagonisten Nifedipin und Nisoldipin mit dem nichtdepolarisierenden Muskelrelaxans Vecuronium-Bromid im “in vivo” Rattenpräparat. Der Anästhesist34, in press (1985).Google Scholar
  10. Israel, J. M., Meunier, J. M.: A calcium dependent acetylcholine depolarization blocked by Methoxyverapamil (D600) and procaine in snail neurones. Br. J. Pharmacol.68, 235–240 (1980).PubMedGoogle Scholar
  11. Jenkins, L. C., Scoates, P. J.: Anesthetic implications of calcium channel blockers. Can. Anaest. Soc.32, 436–447 (1985).Google Scholar
  12. Kraynack, B. J., Lawson, N. W., Gintautas, J., Hon Tjoenj Tjay. Anesth. Analg.62, 827–830 (1983).PubMedGoogle Scholar
  13. Llinás, R., Steinberg, I. Z., Walton, K.: Presynaptic calcium currents in squid giant synapse. Biophys. J.33, 289–321 (1981).PubMedGoogle Scholar
  14. Peracchia, C., Bernardini, G.: Gap junction structure and cell-to-cell coupling regulation: is there a calmodulin involvement? Federation Proc.43, 2681–2691 (1984).Google Scholar
  15. Loewe, S., Muischnek, H.: Über Kombinationswirkungen. Arch. Exp. Pathol. Pharmacol.114, 313–326 (1926).Google Scholar
  16. Miledi, R., Parker, I.: Blocking of acetylcholine induced channels by extra cellular or intracellular application of D600. Proc. R. Soc. (Lond.)B 211, 143–150 (1980).Google Scholar
  17. Publicover, St. J., Duncan, Ch. J.: The action of Verapamil on the rate of spontaneous release of transmitter at the frog neuromuscular junction. Eur. J. Pharmacol.54, 119–127 (1979).PubMedGoogle Scholar
  18. Rang, H. P.: Drugs and ionic channels: mechanism and implications, postgrad. Med. J. 57 (Suppl.), 89–97 (1981).Google Scholar
  19. Reves, J. C., Kissin, I., Lell, W. A., Tosone, St.: Calcium entry blockers: uses and implications for anesthesiologists. Anesthesiology57, 504–518 (1982).PubMedGoogle Scholar
  20. Sato, T., Ono, H.: Facilitation of neuromuscular transmission by calcium antagonists Diltiazem, Nifedipine and Verapamil in the dog. Arch. Int. Pharmacodyn. Ther.249, 233–246 (1981).Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • W. Ilias
    • 1
  • K. Steinbereithner
    • 1
  1. 1.L. Boltzmann-Institut für Experimentelle Anaesthesiologie und Intensivmedizinische ForschungKlinik für Anaesthesie und Allgemeine Intensivmedizin der Universität WienÖsterreich

Personalised recommendations