Radiation and Environmental Biophysics

, Volume 33, Issue 1, pp 69–79 | Cite as

Osteosarcomagenic doses of radium (224Ra) and infectious endogenous retroviruses enhance proliferation and osteogenic differentiation of skeletal tissue differentiating in vitro

  • Jörg Schmidt
  • Kathrin Heermeier
  • Utz Linzner
  • Arne Luz
  • Michael Silbermann
  • Erella Livne
  • Volker Erfle


Cartilage tissue from embryonic mice which undergoes osteogenic differentiation during in vitro cultivation was used to study the effect of osteosarcomagenic doses of α-irradiation and bone-tumor-inducing retroviruses on proliferation and phenotypic differentiation of skeletal cells in a defined tissue culture model. Irradiated mandibular condyles showed dose-dependent enhancement of cell proliferation at day 7 of the culture and increased osteogenic differentiation at day 14. Maximal effects were found with 7.4 Bq/ml of224Ra-labeled medium. Doses of 740 and 7400 Bq/ml of224Ra-labeled medium induced increasing cell death. Retrovirus infection enhanced osteogenic differentiation and extended the viability of irradiated cells. After transplantation none of the treated tissues developed tumors in syngeneic mice.


Cell Death Cell Proliferation Radium Tissue Culture Environmental Physic 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Jörg Schmidt
    • 1
  • Kathrin Heermeier
    • 1
  • Utz Linzner
    • 2
  • Arne Luz
    • 2
  • Michael Silbermann
    • 3
  • Erella Livne
    • 3
  • Volker Erfle
    • 1
  1. 1.Institut für Molekulare VirologieGSF-Forschungszentrum für Umwelt und Gesundheit GmbHOberschleißheimGermany
  2. 2.Institut für PathologieGSF-Forschungszentrum für Umwelt und Gesundheit GmbHOberschleißheimGermany
  3. 3.Laboratory of Musculoskeletal ResearchThe Rappaport Family Institute for Research in the Medical Sciences, The Bruce Rappaport Faculty of Medicine, TechnionHaifaIsrael

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