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KontraktilitÄt und Ultrastruktur glycerin-extrahierter Fibroblasten aus der Gewebekultur

Contractility and ultrastructure of glycerol-extracted fibroblasts in tissue culture

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Zusammenfassung

Hühnerherz-Fibroblasten der Wachstumszone von Herzexplantaten 9 Tage alter Embryonen wurden 1/2 Std. 1 und 12 Tage lang glycerinextrahiert. (d-)Filamente mit einem Durchmesser von 60–80 Ä, die überall im Cytoplasma vorhanden sind, und au\erdem feinere (f-)Filamente mit einem Durchmesser von 20–30 å in der Randzone der Zellen überstehen die Extraktion. Gelegentlich sind einige d-Filamente in Subfilamente mit einem Durchmesser von 20–30 å aufgetrennt. Die durch ATP erzeugte Kontraktion bewirkt eine Abrundung der Zelle. Ein elektronendichtes Material lagert sich dabei schalenförmig um die Zelle. Dieses Material besteht aus d- und f-Filamenten wie die Randzone nicht-extrahierter Zellen. Das durch ATP erzeugte elektronendichte Material und die Randzone scheinen daher der kontrahierte Zustand und die im Cytoplasma verteilten d-Filamente der nicht-kontrahierte Zustand eines intrazellulÄren kontraktilen Systems zu sein.

Summary

Chick heart fibroblasts outgrown from heart explants of nine-day embryos were glycerol-extracted for 1/2 hour, 1 and 12 days. (d-)filaments with a diameter of 60–80 å existing everywhere in the cytoplasma and in addition finer (f-)filaments with a diameter of 20–30 å in the cortical layer of the cells withstand the extraction. Occasionally some d-filaments have divided into subfilaments with a diameter of 20–30 å. Contraction induced by ATP results in a rounding up of the fibroblast. An electron dense material is found in a shell-like arrangement around the condensed cell. This material consists of d- and f-filaments just like the cortical layer of unextracted cells. The ATP-induced electron dense material and the cortical layer are supposed to be the contracted and the d-filaments dispersed in the cytoplasma the relaxed state of an intracellular contractile system.

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Author notes

  1. D. Graf Keyserlingk

    Present address: II. Anatomisches Institut der Freien UniversitÄt Berlin, Deutschland

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    Keyserlingk, D.G. KontraktilitÄt und Ultrastruktur glycerin-extrahierter Fibroblasten aus der Gewebekultur. Protoplasma 67, 391–406 (1969). https://doi.org/10.1007/BF01254903

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