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The formation of wall-like envelopes by isolated tomato-fruit protoplasts

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Summary

Formation of a new cell wall around tomato protoplasts was confirmed by optical microscopy, electron microscopy and X-ray diffraction. This wall is composed of three layers; (a) an outer ring, which seems to be composed of diffuse, amorphous material, (b) an intermediate space, crossed by radial fibers, (c) a thicker, inner band composed of dense, highly consolidated material which may have sub-layers within it. Occasionally, cells are observed with only the dense consolidated layer about them. The origin of this wall and its component layers is not yet understood.

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Author notes

  1. J. Ross Colvin

    Present address: Division of Biochemistry and Molecular Biology National Research Council of Canada, Ottawa, Canada

Authors and Affiliations

  1. Division of Biochemistry and Molecular Biology National Research Council of Canada, Ottawa, Canada

    A. K. Mishra

Authors
  1. A. K. Mishra
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  2. J. Ross Colvin
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National Research Council Post-doctoral Fellow, 1967–1969.

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Mishra, A.K., Colvin, J.R. The formation of wall-like envelopes by isolated tomato-fruit protoplasts. Protoplasma 67, 295–305 (1969). https://doi.org/10.1007/BF01254895

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  • DOI: https://doi.org/10.1007/BF01254895

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